대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제28권2호
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- Pages.175-182
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- 1998
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- 1225-2344(pISSN)
한국인에서 Hepatitis G Virus (HGV) 검출 및 항원분석에 관한 연구
The Detection and the Antigenic Analysis of the Hepatitis G Virus in Korea
- 윤재득 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 지영미 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 이홍래 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 김기순 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 김영선 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 이윤성 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 정윤석 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 박정구 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 김지은 (국립보건원 바이러스질환부 소화기계바이러스과) ;
- 정상인 (중앙대학교 의과대학) ;
- 이원선 (부천 성가병원) ;
- 이원배 (부천 성가병원)
- Yoon, Jae-Deuk (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Jee, Young-Mee (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Lee, Hong-Rae (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Kim, Ki-Soon (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Kim, Young-Sun (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Lee, Yoon-Sung (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Chung, Yoon-Suk (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Park, Jeong-Koo (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Kim, Ji-Eun (Laboratory of Enteroviruses, Department of Viral Diseases, National Institute of Health) ;
- Chung, Sang-In (Department of Microbiology, Medical College, Chung-Ang University) ;
- Lee, Won-Sun (Holy Family Hospital, Bucheon City) ;
- Lee, Won-Bae (Holy Family Hospital, Bucheon City)
- 발행 : 1998.06.30
초록
We investigated the rate of hepatitis G virus infection among 50 patients who were not infected with the hepatitis C virus but showed symptoms of hepatitis. Viral RNA was extracted from the patients' sera and cDNA was synthesized and amplified by RT-PCR (reverse transcription-polymerase chain reaction) using random hexamer and 5 primers (470-20-1-77F, 470-20-1-211R, 470-20-1-211R-biotin, GV57-4512MF, GV57-4657MR). The amplified PCR products were confirmed by electrochemiluminescence (ECL), liquid hybridization (LH) and Southern blotting (SB). Among the 50 PCR products, by means of ECL, we found 4 samples to be positive and 5 samples to be indeterminate. The GV45-89M probe (5'-CYCGCTGRTITGGGGTGTACfGGAAGGC-3') was end-labelled with gamma-
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