The Korean Journal of Nuclear Medicine (대한핵의학회지)
- Volume 32 Issue 6
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- Pages.516-524
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- 1998
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- 1225-6714(pISSN)
In Vitro Properties and Biodistribution of Tc-99m and Re-188 Labeled Monoclonal Antibody CEA79.4
Re-188과 Tc-99m 표지 단일클론항체 CEA79.4의 생체외 특성과 생체내 분포
- Hong, Mee-Kyoung (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Jeong, Jae-Min (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Yeo, Jeong-Seok (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Kim, Kyung-Min (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Chang, Young-Soo (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Lee, Yong-Jin (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Lee, Dong-Soo (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Chung, June-Key (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Lee, Myung-Chul (Department of Nuclear Medicine, Seoul National University College of Medicine) ;
- Lee, Seung-Jin (Ewha Woman University College of Pharmacy)
- 홍미경 (서울대학교 의과대학 핵의학교실) ;
- 정재민 (서울대학교 의과대학 핵의학교실) ;
- 여정석 (서울대학교 의과대학 핵의학교실) ;
- 김경민 (서울대학교 의과대학 핵의학교실) ;
- 장영수 (서울대학교 의과대학 핵의학교실) ;
- 이용진 (서울대학교 의과대학 핵의학교실) ;
- 이동수 (서울대학교 의과대학 핵의학교실) ;
- 정준기 (서울대학교 의과대학 핵의학교실) ;
- 이명철 (서울대학교 의과대학 핵의학교실) ;
- 이승진 (이화여자대학교 약학대학)
- Published : 1998.12.30
Abstract
Purpose: Radiolabeled CEA79.4 antibody has a possibility to be used in radioimmunoscintigraphy or radioimmunotherapy of cancer. We investigated the in vitro properties and biodistribution of CEA79.4 antibody labeled with Re-188 or Tc-99m. Materials and Methods: CEA79.4 was reduced by 2-mercaptoethanol to produce-SH residue, and was labeled with Re-188 or Tc-99m. For direct labeling of Tc-99m, methylene-diphosphonate was used as transchelating agent. CEA79.4 in 50 mM Acetate Buffered Saline (ABS, pH 5.3) was labeled with Re-188, using stannous tartrate as reducing agent. In order to measure immunoreactivity and the affinity constant of radiolabeled antibody, cell binding assay and Scatchard analysis using human colon cancer cells SNU-C4, were performed. Biodistribution study of labeled CEA79.4 was carried out at 1, 14 and 24 hr in ICR mice. Results: Labeling efficiencies of Tc-99m and Re-188 labeled antibodies were
목적: 현재 Re-188 표지 단일클론항체는 종양의 방사면역치료제로서 주목받고 있으며 많은 연구가 진행되고 있다. 이 연구에서는 골수성 백혈구 세포에 존재하는 NCA-95에 대한 단일클론항체 CEA79.4를 사용하여 Re-188의 항체 표지법을 개발하고 그 특성을 분석하여 Tc-99m 표지항체와 비교하고자하였다. 대상 및 방법: 표지는 항체에 방사성 핵종을 직접 붙이는 직접법을 사용하였다. 환원제인
Keywords
- Direct labeling method;
- Re-188;
- antibody;
- Radioimmunotherapy;
- Generator;
- Immunoreactivity;
- Affinity constant