Clinical and Experimental Reproductive Medicine
- 제25권2호
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- Pages.207-216
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- 1998
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
체세포배양액이 생쥐 난자의 Chymotrypsin에 대한 내성에 미치는 영향
Effects of Somatic Cell Conditioned Medium on the Chymotrypsin Resistance of Mouse Oocytes
- 김성례 (이화여자대학교 의과대학 의학과 및 의과학연구소 분자생물학부) ;
- 정혜원 (이화여자대학교 의과대학 산부인과학교실 및 의과학연구소 분자생물학부) ;
- 김성임 (이화여자대학교 의과대학 의학과 및 의과학연구소 분자생물학부) ;
- 김해권 (서울여자대학교 생물학과)
- Kim, Sung-Rye (Department of Medicine, Division of Molecular, Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University) ;
- Chung, Hye-Won (Department of Obstetrics & Gynecologt, Division of Molecular Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University) ;
- Kim, Seong-Im (Department of Medicine, Division of Molecular, Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University) ;
- Kim, Hae-Kwon (Department of Biology, Seoul Women's University)
- 발행 : 1998.06.30
초록
Certain types of somatic cells, as well as follicular cumulus cells associating with mammalian oocytes, are known to produce beneficial effects on in vitro fertilization and pre implantation development of mammalian eggs when they are present in oocyte culture medium. To investigate the nature of the effects of somatic cells, the resistance of mouse oocytes against chymotrypsin treatment was examined after culture within various cell conditioned media. When mouse oocytes matured for 17-18 hr in the presence of cumulus cells were treated with 1 % chymotrypsin, half of them remained still alive even after 240 min