Clinical and Experimental Reproductive Medicine
- Volume 25 Issue 2
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- Pages.171-177
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- 1998
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Efficacy and Fertilizing Ability of Frozen-thawed Testicular Spermatozoa and Spermatozoa Extracted from the Seminiferous Tubule with Intracytoplasmic Sperm Injection (ICSI)
동결-융해 후 회수된 고환 정자와 세정관내 정자의 수정 능력과 효율성에 관한 연구
-
Park, Yong-Seog
(Infertility Research Laboratory, Samsung Cheil Hospital & Women's Healthcare Center) ;
- Jun, Jin-Hyun (Infertility Research Laboratory, Samsung Cheil Hospital & Women's Healthcare Center) ;
- Lee, Ho-Joon (Infertility Research Laboratory, Samsung Cheil Hospital & Women's Healthcare Center) ;
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Kang, Inn-Soo
(Department of Obstetrics and Gynecology, Samsung Cheil Hospital & Women's Healthcare Center) ;
- Kim, Jong-Hyun (Department of Urology, Samsung Cheil Hospital & Women's Healthcare Center) ;
- Lee, You-Sik (Department of Urology, Samsung Cheil Hospital & Women's Healthcare Center) ;
- Seo, Ju-Tae (Department of Urology, Samsung Cheil Hospital & Women's Healthcare Center)
-
박용석
(삼성제일병원 불임연구실) ;
- 전진현 (삼성제일병원 불임연구실) ;
- 이호준 (삼성제일병원 불임연구실) ;
-
강인수
(삼성제일병원 산부인과) ;
- 김종현 (삼성제일병원 비뇨기과) ;
- 이유식 (삼성제일병원 비뇨기과) ;
- 서주태 (삼성제일병원 비뇨기과)
- Published : 1998.06.30
Abstract
The combination of testicular sperm extraction (TESE) with ICSI can achieve normal fertilization and pregnancy rate and is effective method in obstructive and non-obstructive azoospermic patients. But, when pregnancy was not occurred, repeated testicular biopsies are not evitable. Therefore, in this study, we observed the survival rate of testicular spemratozoa and spermatozoa extracted from the seminiferous tubules after cryopreserved-thawed used for next IVF cycle with ICSI. In a total of 23 cases, obstructive azoospermia was 17 cases and non-obstructive azoospermia was 6 cases. In obstructive azoospermia, after thawing, motile spermatozua was observed in 13 cases (76.5%). The fertilization rate with 2PN was 67.6% and 5 pregnancies (29.4%) were achieved. In non-obstructive azoospermia, motile spermatozoa was observed in 2 case (33.3%) after thawing. The fertilization rates with 2PN was 53.7% and 3 pregnancies (50.0%) were achieved. A comparison of the results of motile spermatozoa after thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section were 3 cases (60.0%) and 12 cases (66.6%), respectively. The fertilization and pregnancy rates of thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section were 69.4% and 20.0%, 62.5% and 38.8%, respectively. Conclusively, thawed testicular spermatozoa and spermatozoa extracted from the thawed seminiferous tubule section can achieve normal fertilization and pregnancy and cryopreservation of testicular spermatozoa and seminiferous tubule may avoid repetition of testicular biopsies in azoospermic patients in whom the only source of spermatozoa is the testis.