Clinical and Experimental Reproductive Medicine
- 제25권3호
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- Pages.315-322
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- 1998
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
돼지정자의 수정능력과 Reactive Oxygen Species의 관계분석 I. Xanthine과 Xanthine Oxidase에 의한 정자의 전배양
Analysis of Relationship Between Spermatozoa Ability and Reactive Oxygen Species in Porcine: I. Sperm Preincubation by Xanthine and Xanthine Oxidase
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박춘근
(강원대학교 축산대학) ;
-
정희태
(강원대학교 축산대학) ;
- 김종흥 (세화산부인과 불임크리닉) ;
- 이상찬 (세화산부인과 불임크리닉) ;
- 양부근 (강원대학교 축산대학) ;
- 김정익 (강원대학교 축산대학)
-
Park, C.K.
(College of Animal Agriculture, Kangwon University) ;
-
Cheong, H.T.
(College of Animal Agriculture, Kangwon University) ;
- Kim, J.H. (Saewha Infertility Clinic) ;
- Lee, S.C. (Saewha Infertility Clinic) ;
- Yang, B.K. (College of Animal Agriculture, Kangwon University) ;
- Kim, C.I. (College of Animal Agriculture, Kangwon University)
- 발행 : 1998.09.30
초록
본 연구는 xanthine과 xanthine oxidase가 첨가된 배양액내에서 전배양된 돼지동결정액의 수정능력에 있어서 catalase의 영향을 검토하였다. 체외수정을 위한 기본 배양액내에서 0 또는 30분간 전배양한 정자는 catalase를 첨가 (40 및 15%)한 경우 보다는 무첨가 (66 및 38%)시 유의적으로 높은 정자침입율을 나타냈지만 (p<0.05), 배양액내에 xanthine을 첨가해 정자를 0, 30 및 60분간 전배양했을 때에는 catalase 무첨가 (33, 41 및 19%) 보다는 첨가시 (68, 70 및 49%) 유의적으로 높은 정자침입율이 인정되었다 (p<0.05). 그러나 xanthine oxidase를 첨가하여 정자의 전배양을 행하지 않은 경우는 catalase의 첨가 (13%) 보다는 무첨가 (51%)시 유의적으로 높은 정자침입율을 나타냈지만 (p<0.01), 전배양(30 및 60분)후에는 catalase의 존재유무와 정자전배양시간에 관계없이 매우 낮은 정자침입율
The objective of this study was to test the effect of catalase on penetration in vitro by spermatozoa preincubated with xanthine and/or xanthine oxidase. The penetration rates were, significantly (p<0.05) higher in spermatozoa preincubated without (66 and 38%) than with (40 and 15%) catalase for 0 and 30 min. When spermatozoa were preincubated and inseminated in medium with xanthine, the penetration rates were significantly higher (p<0.05) in medium with (68, 70 and 49% for 0, 30 and 60 min) than without (33, 41 and 19% for 0, 30 and 60 min) catalase. However, in oocytes were' inseminated with spermatozoa pre incubated with or without catalase in the presence of xanthine oxidase, no decrease in penetrations rates were observed for up to 60 min of preincubation. In another experiment, the penetration rates were significantly (p<0.00l) higher in medium with (75, 55 and 52%) than without (14, 4 and 8%) catalase when oocytes were inseminated with spermatozoa preincubated for 0, 30 and 60 min in the presence of xanthine plus xanthine oxidase. On the other hand, The rate of polyspermy in oocytes penetrated in medium without catalase in the presence of xanthine or xanthine plus xanthine oxidase decreased with time of spermatozoa preincubation. However, no differences were observed in polyspermy rates in the medium with xanthine oxidase alone despite presence of catalase. These results indicate the advantages of spermatozoa pre incubated with xanthine plus xanthine oxidase in the presence of catalase to increase penetration potential and with suppressed polyspermy in porcine.