Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
권호 표지

Characteristics of Acetone Enhancement of Microsomal Cytochrome P45O-dependent B(a)P Hydroxylation in 3-Methylcholanthrene-inducible Rat Liver Microsomes

  • Lee, Dong-Wook (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute) ;
  • Moon, Ja-Young (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute) ;
  • Lim, Heung-Bin (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute) ;
  • Sohn, Hyung-Ok (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute) ;
  • Lee, Young-Gu (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute) ;
  • Park, Ki-Hyun (Laboratory of Biochemistry, Korea Ginseng and Tobacco Research Institute)
  • Published : 1998.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Previously, we showed that acetone enhanced aryl hydrocarbon hydroxylase (AHH) activity in only 3-methylcholanthrene (MC)- or $\beta$-naphtoflavone (BNF)-inducible microsomes of rat liver. In the present study, the possible mechanism underlying acetone action on AHH was investigated in the liver microsomes from MC-pretreated rats. Other n-alkylketones except acetone did not increase AHH activity, which rather decreased significantly with the length of alkyl side chain. Acetone had no effect on the activity of NADPH-cytochrome P450 reductase or inhibited the formation of 3-OH benzo(a)pyrene (B(a)P) in nonenzymatic model ascorbic acid system. However, in cumene hydroperoxide (CuOOH)-supported B(a)P hydroxylation, acetone enhanced its velocity remarkably by 30% at the optimal concentration (30 $\mu$M CuOOH and 1.0% acetone). From these results, we conclude that acetone may facilitate the formation of an activated oxygen species or the insertion of oxygen into B(a)P molecule in CYP1A rich microsomes.

Keywords