Production of Cellulase by Trichoderma reesei Rut C30 in a Batch Fermenter

  • Yu, Xiao-Bin (School of Biotechnology, Wuxi University of Light Industry) ;
  • Yun, Hyun-Shik (Department of Biological Engineering, Inha University) ;
  • Koo, Yoon-Mo (Department of Biological Engineering, Inha University)
  • Published : 1998.12.01

Abstract

Cellulase production by batch culture using the Trichoderma reesei Rut C30 strain with various concentrations of Solka Floc with 1 % wheat bran was studied in a 2.5 I fermenter. The cellulase activity increased with Solka Floc concentration up to 5%. When 5% Solka Floc and 1% wheat bran were contained in the medium, carboxymethyl cellulose (CMC) and filter paper (FP) activities were 232.4 U/$m\ell$ and 21.25 U/$m\ell$, respectively. The productivity was 143.6 FPU $1^{-l}h^{-1}$ and the yield was 425 FPU/g. The colonial morphology of T. reesei Rut C30 grown on Avicel agar plates and the changes in mycelial morphology of T. reesei Rut C30 with culture time are also presented.

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