Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

A Pathway for 4-Chlorobenzoate Degradation by Pseudomonas sp. S-47

  • Seo, Dong-In (Department of Microbiology, Chungbuk National University) ;
  • Chae, Jong-Chan (Department of Microbiology, Chungbuk National University) ;
  • Kim, Ki-Pil (Department of Microbiology, Chungbuk National University) ;
  • Kim, Young-Soo (Department of Pharmacy, Chungbuk National University) ;
  • Lee, Ki-Sung (Department of Biology, Pai-Chai University) ;
  • Kim, Chi-Kyung (Department of Microbiology, Chungbuk National University)
  • Published : 1998.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Pseudomonas sp. S-47 degraded 4-chlorobenzoate (4CBA) to 4-chlorocatechol (4CC) that was subsequently ring-cleaved to form 5-chloro-2-hydroxymuconic semialdehyde. These intermediate compounds were identified by GC-mass spectrometry and UV-visible spectrophotometry. 5-chloro-2-hydroxymuconic acid converted from 5-chloro-2- hydroxymuconic semialdehyde (5C-2HMS) was dechlorinated to produce 2-hydroxypenta-2,4-dienoic acid (2HP-2,4DA) by the strain. These results indicate that Pseudomonas sp. S-47 degrades 4CBA to 2HP-2,4DA via a novel pathway including the meta-cleavage of 4CC and dechlorination of 5C-2HMS.

Keywords