Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration from Cryopreserved Embryogenic Cell Suspension Cultures of Cucumber

초저온 보존된 오이 배발생세포 현탁배양으로부터 식물체 재분화

  • Kim, Seok-Won (Gene Bank, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • In, Dong-Soo (Division of Biotechnology, Plant Cell & Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jung, Won-Joong (Division of Biotechnology, Plant Cell & Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Woo, Je-Wook (Division of Biotechnology, Plant Cell & Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Jung, Min (Division of Biotechnology, Plant Cell & Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Yoo, Jang-Ryul (Division of Biotechnology, Plant Cell & Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김석원 (생명공학연구소 유전자원센터 유전자은행사업실) ;
  • 인동수 (생명공학연구소 유전자원센터 제2연구부 식물세포 및 분자생물학 R.U.) ;
  • 정원중 (생명공학연구소 유전자원센터 제2연구부 식물세포 및 분자생물학 R.U.) ;
  • 우제욱 (생명공학연구소 유전자원센터 제2연구부 식물세포 및 분자생물학 R.U.) ;
  • 정민 (생명공학연구소 유전자원센터 제2연구부 식물세포 및 분자생물학 R.U.) ;
  • 유장렬 (생명공학연구소 유전자원센터 제2연구부 식물세포 및 분자생물학 R.U.)
  • Published : 1998.11.01
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Abstract

Conditions for high frequency plant regeneration from cryopreserved embryogenic cell suspension cultures derived from hypocotyl explants of cucumber (Cucumis sativus L.) are described. Cells cryoprotected with a mixture of 2 M DMSO and 0.4 M sucrose exhibited a regeneration frequency of 85%. However, cells cryoprotected with different concentrations of glycerol showed no regeneration after cryopreservation. Pretreatment of cells in a high osmotic medium was not necessary to the process. Upon transfer to MS medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid, regenerated calli gave rise to numerous somatic embryos, then underwent development into plantlets.

오이의 국내 F1 품종인 조생낙합의 하배축 유래 배발생 현탁배양 세포의 초저온 보존 시스템을 개발하였다. 액체질소 저장 후 캘러스 재생률은 2M DMNSO와 0.4 M sucrose를 혼용 처리하였을 때 캘러스 재생률이 85%로 가장 높았다. 그러나 glycerol 처리구에서는 처리농도에 상관없이 모든 처리구에서 캘러스 재생이 이루어지지 않았다. 또한 고농도의 삼투용액에서 배양세포의 전처리 과정은 필요하지 않았다. 재생된 캘러스를 1 mg/L 2,4-D가 첨가된 MS 배지로 이식하여 배양하였을 때 다수의 체세포배가 발달하였으며, 체세포배를 MS 기본배지로 옮겨 명배양한 결과 다수의 소식물체가 발달하였다.

Keywords

References

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