Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Incubation Time, Concentration of Enzyme, and 2,4-D on Isolation and Callus Formation of Protoplast from Callus of Citrus junos

遊離시간 , 酵素處理 및 2,4-D 농도가 재래 유자(Citrus junos)의 캘러스由來 原形質體 遊離 및 培養에 미치는 영향

  • Published : 1998.09.01
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Abstract

The factors affecting the isolation and culture of the protoplast of embryogenic callus, derived from immature ovule in Citrus junos, were examined. An incubation time in enzyme solution of 16 hrs was preferable for protoplast isolation. Efficient protoplast yields were obtained from the treatment of equal concentration of 0.7 M $\textrm{BH}_{3}$ to the enzyme solution containing 1.0% cellulase, 1.0% macerozyme and 0.2% pectolyase. Protoplast cultured in MT medium with 0.1 mg/L 2,4-D showed vigorous division and some of them formed callus. Induced callus was subcultured on solid MT medium but the callus showed very slow growth. The above results show the possibility to culture from protoplast fusion in Citrus genera.

미숙배주조직 유래의 배발생캘러스를 이용하여 원형질체의 유리 및 배양에 미치는 요인을 조사하였다. 배발생캘러스로부터 원형질체를 유리시키는데 적당한 배양시간은 16시간이었고, 건전한 원형질체를 유리하는데 적당한 효소용액의 농도는 0.7M $\textrm{BH}_{3}$ 용액과 cellulase 1.0%, macerozyme 1.0%, pctolyase 0.2%가 혼합된 효소용액을 동량으로 조합하였을 때가 가장 효과적이었다. 유리된 원형질체는 MT기본배지에 0.1 mg/L 2,4-D를 첨가한 배지로 배양하면 캘러스 형성이 양호하였다. 유도된 캘러스는 고체배지에 계대배양하고 있으나 생육이 징약한 상태이다. 본 실험결과 배발생캘러스유래의 원형질체와 엽육세포유래의 원형질체 융합에 의한 배양도 가능할 것으로 생각되었다.

Keywords

References

  1. sprengeri. Plant Cell Reports v.17 Factors influencing regeneration from protoplasts of Asparagus densiflorus cv. Benmoussa M;Mukhopadhyay S;Desjardins Y
  2. J Kor Soc Hort Sci v.27 Isolation and culture of protoplast of omamental tomato(Lycopersion esculentum x L. pimpinelifolium cv. Tiny Tim) Chung JD;Lee MH;Chun CK
  3. Theor Appl Genet v.80 Induction of triploid Citrus plants from endosperm calli in vitro Gmitter Jr FG;Ling XB;Deng XX
  4. Sci Hort v.31 Aseptic isolation of leaf protoplasts from Citrus, Poncirus, Citrus x Proncirus hybrids and Severinia for use in somatic hybridization experiments Grosser JW;Chandler JL
  5. Plant Breed Rev v.8 Protoplast fusion and Citrus improvement Grosser JW;Gmitter Jr FG
  6. HortScience v.25 Somatic hybridization of Citrus with wild relatives for germplasm enhancement and cultivar development Grosser JW;Gmitter Jr FG
  7. Hort Biotech : Wide Hybridization of Citrus via protoplast fusion: Progress, strategies, and limitations Grosser JW;Gmitter Jr FG
  8. Theor Appl Genet v.75 Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrussinensis and Severinia disticha Grosser JW;Gmitter Jr FG;Chndler JL
  9. HortScience v.27 Production of somatic hybrid and autotetraploid breeding parents for seedless Citrus development Grosser JW;Gmitter Jr FG;Loudaza ES;Chndler JL
  10. Plant Cell Reports v.15 Further evidence of a cybridization requirement for plant regeneration from Citrus leaf protoplasts following somatic fusion Grosser JW;Gmitter Jr FG;Tusa N;Reforgiato Recupero G;Cucinotta P
  11. HortScience v.29 Somatic hybridization of complementary citrus rootstocks: five new hybrids Grosser JW;Louzada ES;Gmitter Jr FG;Chandler JL
  12. Japan J Breed v.42 Regeneration of somatic hybrid plants obrained by electrical fusion between satsuma mandarine(Citrus Unshin) and rough lemon(C. jambhiri) or yuzu(C. junos) Hidaka T;Omura M
  13. Korean J Plant Tissue Culture v.19 Protoplast isolation and culture of grapes cv. 'Camernet sauvignon'(Vitis vinifera L.) and 'Niagara'(Vitis labrusca L.) Hyung NI;Kang SK;Lee CH
  14. Plant Cell Reports v.15 Plant regeneration via somatic embryogenesis from protoplasts of Urganda cherry orange(Citropsis schwenfurthii) Jumin HB;Nito N
  15. Plant Cell Reports v.17 Plant regeneration from leaf protoplasts of evening primrose(Oenothera hookeri) Kuchuk N;Herrman RG;Koop HU
  16. Sci Hort v.40 Plant regeneration from protoplasts of Calamondin (Citrus madrensis Lour.) Ling JT;Nito N;Iwamasa M
  17. J Japan Soc Hort Sci v.65 Protoplast fusion for production of hybrid plants between Citrus and its related genera Motomura T;Hidaka T;Akihawa T;Tatagi S;Berhow M;Moriguchi T;Omura M
  18. Proc 1st International Citrus Symposium v.3 Growth factor requirements of citrus tissue culture Murashige T;Tucker DPH;HD Champman(ed.)
  19. J Kor Soc Hort Sci v.32 In vitro micropropagation of yooza(Citrus junos Sieb et Tanaka). I. Plant regeneration from callus induced from shoot tips Oh SD;Song WS;Kim JS;Park EH
  20. Theor Appl Genet v.71 Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata Ohgawara T;Kobayashi S;Ohgawara E;Ishii S
  21. Plant Cell Reports v.7 Direct embryogenesis from protoplasts of Citrus mitis Blanco Sim GE;Loh CS;Goh CJ
  22. Sci Hort v.37 Recent advances in protoplast culture of horticultural crops:Citrus. Vardi A;Galun E
  23. Plant Science Letters v.4 Citrus cell culture: isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos Vardi A;Spiegel-Roy P;Galum E
  24. Theor Appl Genet v.62 Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species Vardi A;Spiegel-Roy P