Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration from Leaf and Petiole Culture of Kiwifruit(Actinidia deliciosa)

참다래(Actinidia deliciosa)의 엽 및 엽병배양에 의한 식물체 재분화

  • Published : 1998.09.01
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Abstract

Leaf and petiole explants of kiwifruit were cultured on MT basal medium supplimented with 2,4-D, kinetin, NAA, and BA. Higher organogenic callus formation was observed on the media with NAA + BA than on the media added with 2,4-D + kinetin. Adventitious buds were formed only on media with NAA and BA. Leaf was better explant than petiole. When callus and adventitious buds were subcultured, shoot formation responsed best on medium with 0.1 mg/L NAA + 2.0 mg/L zeatin. When shoots were cultured on medium with 0.5 mg/L IAA + 0.1 mg/L BA after soaking for 1 hr at IBA solution, rooting was more effective than non-IBA treatment. Rooted shoots developed into normal plants.

키위의 엽육조직과 엽병조직을 치상하여 캘러스 및 부정아형성에 미치는 생장조절제의 효과를 조사한 결과 기관분 화성 캘러스형성은 2,4-D와 kinetin을 조합한 배지에서보다 NAA와 BA를 조합한 배지에서 양호하였고, 부정아 형성은 NAA와 BA를 조합한 배지에서만 형성되었으며 엽병조직보다 엽육조직을 배양하였을 때 반응이 양호하였다. 유도된 캘러스와 부정아로부터 신초의 형성은 MS기본배지에 0.1mg/L NAA와 2.0mg/L zeatin을 첨가한 배지에 계대배양하였을 때가 가장 효과적이었다. 신초의 발근은 1.0 mg/L IBA용액에 1시간 신초를 침지한 후 0.5 mg/L IAA와 0.1mg/L BA를 첨가한 배지에 배양하면 IBA용액에 침지처리 하지 않은 것보다 더 효과적이었으며 정상식물체로 발달하였다.

Keywords

References

  1. Plant Physiol v.136 Restoration of the regeneration potential of long term cell culture in rice(Oryza sativa) by salt pretreatment Binh OQ;Heszky LE
  2. Actinindin tissue cultures. Plant Cell Reports v.16 Relationship between endogenous auxin and cytokinin level and morphogenic response Centeno ML;Rodrigney A;Feito I;Fermandey B
  3. Plant Sci v.67 Embryogenesis and regeneration from microspones of both indica and japonica rice(Oryza sativa) Data SK;Data K;Potrycas I
  4. Theor Appl Genet v.59 Somatic embryogenesis and plant regeneration from leaf tissue and anthers of Pennisetum purpureum Schum Haydu Z;Vasil IK
  5. Plant Cell Reports v.9 High efficienty plant regeneration from cotyledons of watermelon(Citrallus valgaris Schrad) Jin ZD;Shi RJ
  6. Plant Cell Reports v.8 Plant regeneration from cell suspension cultures of Arabidopsis thaliana Heynh Gleddie S
  7. Acta Hort v.297 Somaclonal variations related to kiwifruit micropropagation, study of fruitful male plants and use of peroxidase as an earlier sex marker Hirsch AM;Fottune D;Xiao XG;Blanchet P
  8. Vitro. Plant Cell Tissue and Organ Culture v.47 Effects of light, magnesium and sucrose on leaf anatomy, photosynthesis, starch and total sugar accumulation in Kiwifruit cultured Kortessa DT;Artemios MB
  9. Kor J Plant Tissue Culture v.21 Plant regeneration from hypocotyle explants of several species of Lycopersicon Lim HT;Lee KS;Yeoung YR;Song YN;Kim JH
  10. Physiol Plnat v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige T;Skoog F
  11. Proc. 1st International Citrus Symposium v.3 Growth factor requirements of citurs tissue culture Murashige T;Tucker DPH;HD Champman(ed.);
  12. Plant Cell Reports v.9 Plant regeneration from protoplasts of long-term callus cultures of Actinidin deliciosa var. deliciosa cv. Hayword(Kiwifruit) Oliveira MM;Paris MS
  13. Kor J Plant Tissue Culture v.22 Multiple shoot induction from ex vitro and in vitro derived stem node culture of populus alba x P. grandidentata Michx Son SH;Hall RB
  14. Vitro. Plant Cell, Tissue and Organ Culture v.6 Factors affecting plant regeneration from leaf segments of Anthurium scherzerianum Schott (Araceae) cultured Thomas G
  15. Plant Cell Reports v.15 Microcallus formation leaf mesophyll protoplasts in the genes Actinidia Lindl Xiao XG;Hirsch AM
  16. Acta Botanica Sinica v.37 Plantlet regeneration from leaf protoplasts in seedling of Actinidia eriantha Benth Zhang Yj;Mu XJ;Cai QG;Zhou YL;Qian YQ