Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Free Radical Scavenging Activity and Ascorbate Content in Various Plant Cell Lines

다양한 식물배양세포주에서 자유라디칼 포착활성과 Ascorbate 함량

  • AHN, Young-Ock (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • CHOI, Yong-Hwa (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • KWON, Seok-Yoon (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE, Haeng-Soon (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM, Suk-Won (Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • PARK, Il-Hyun (Department of Biochemistry, Chungnam National University) ;
  • KWAK, Sang-Soo (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1998.09.01
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Abstract

We investigated the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity in methanol extracts of 64 cultured cell lines, which were derived from various plant species, and the ascorbate content in cell lines, which showed a high radical scavenging activity. Thirteen cell lines revealed the antioxidative activity ($IC_{50}$) by methanol extracts of less than 50 mg in cell fresh wt. Of them, six cell lines showed the same Rf value as ascorbate on the DPPH sprayed silica gel TLC. The ascorbate content in cell lines of Rosa multiflora, Scutellaria baicalensis, and Achyranthes japonica showed 48.5, 30.3, and $16.8\;\mu\textrm{g}$ per g cell fresh wt by HPLC analysis, respectively. In callus cultures of S. baicalensis, the concentration of ascorbate reached a maximun ($39{\pm}3.4\;\mu\textrm{g}/g$ cell fresh wt) on 30 days after subculture, which corresponded to the stationary growth phase, and subsequently decreased by successive culturing.

다양한 유도조건에서 확립한 64종의 식물배양세포주의 MPA추출물을 대상으로 DPPH 라디칼을 이용하여 항산화 활성을 조사하고 활성이 높은 세포주를 대상으로 HPLC로 ascorbate 함량을 분석하였다. 13종의 세포주는 생체중 50mg 이하에서 DPPH 라디칼의 자연산화를 억제하는 높은 항산화활성을 나타내었다. 이중 6종의 세포주의 MPA추출물을 실리카겔 TLC에 전개하여 DPPH용액을 분무한 결과, ascorbate와 같은 Rf 위치에서 DPPH 양성반응을 나타내는 화합물이 존재하였다. DPPH 양성반응을 나타낸 덩굴장미(Rosa multiflora), 황금(Scutellaria baicalensis), 쇠무릎(Achyranthes japonica) 세포주의 ascorbate 함량($\mu\textrm{g}/g$ cell fresh wt)은 각각 48.5, 30.3, 16.8였다. 황금 캘러스 배양에서 ascorbate의 함량은 세포생장에 비례하여 정지기까지 증가한 후 계속적인 생장에 따라 급격히 감소하였다.

Keywords

References

  1. Antioxidants in Higher Plants Alscher RG;Hess JL
  2. Proc. Natl Acad Sci USA v.93 Environmental stress sensitivity of an ascrobic acid-deficient Arabidopsis mutant Conklin PL;Williams EH;Last RL
  3. Exp Cell Res v.50 Nutrient requirements of suspension cultures of soybean root cells Gamborg OL;Miller RA;Ojima K
  4. J. Chromatogr v.594 Determination of ascorbic and dehydroascorbic acid in potatoes (Solanum tuberosum) and strawberries using ion-exclusion chromatography Graham WD;Annette D
  5. Mol Gen Genet v.255 Molecular cloning and characterization of cDNAs for anionic and neutral peroxidases from suspension-cultured cells of sweet potato and their differential expression in response to stress Huh GH;Lee SJ;Bae YS;Liu JR;Kwak SS
  6. Phytochemistry v.47 Overproduction of sweet potato peroxidases in transgenic tobacco plants Huh GH;Yun BY;Lee HS;Jo JK;Kwak SS
  7. Korean J Plant Tissue Culture v.23 Comparison of catalase and other antixidant enzyme activities in various plant cell lines Jang MS;Huh GH;Kim SW;Park IH;Liu JR;Kwak SS
  8. Korean Biochem J v.27 Selection of plant cell lines for high yields of peroxidase Kim SK;Kwak SS;Jung KH;Min SR;Park IH;Liu JR
  9. Phytochemistry v.39 Acidic peroxidases from suspension-cultures of sweet potato Kwak SS;Kim SK;Lee MS;Jung KH;Park IH;Liu JR
  10. Phytochemistry v.43 Enhancement of peroxidase activity by stress-related chemicals in suspension cultures of sweet potato Kwak SS;Kim SK;Park IH;Liu JR
  11. Abstacts of Keystone Symposium on Metabolic Engineering in Transgenic Plants Genetic approaches to studies of free radical detoxification in Arabidopsis Last RL;Conklin PL;Kliebenstein DJ
  12. Genetic Engineering Research Institute, KIST KCTC Catalogue of Plant Cell Lines Liu JR;Yang SG
  13. Biochem J v.56 Biological synthesis of Lascorbic acid: The conversion of L-galactono-y-lactone into l-ascorbic acid by plant mitochondria Mapson LW;Isherwood FA;Chen YT
  14. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige T;Skoog F
  15. Plant Cell Physiol v.35 L-galactono-r-lactone dehydrogenase: Partial characterization, induction of activity and role in the synthesis of ascorbic acid in wounded white potato tuber tissue Oba K;Fukui M;Imai Y;Iriyama S;Nogami K
  16. J Biochem v.117 Purification and properties of L-galactono-r-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots Oba K;Ishikawa S;Nishikawa M;Mizuno H;Yamamoto T
  17. J Biol Chem v.272 Isolation of a cDNA coding for L-galactono-r-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants Ostergaard J;Persiau G;Davey MW;Bauw G;Montagu MV
  18. Can J Bot v.50 Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell culture Schenk FV;Hildebrandt AC
  19. Biosci Biotech Biochem v.58 A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and sellfish Takao T;Kitatani F;Watanabe N;Akihito Y;Sakata K
  20. Biol Pharm Bull v.19 Antioxidative effects of phenylethanoids from Cistanche deserticola Xiong Q;Kadota S;Tadata T;Namba T
  21. Korean J Plant Tissue Culture v.23 Selection and isoenzyme analysis of plant cell lines for high yields of superoxide dismufase You SH;Kim SW;Kim SH;Liu JR;Kwak SS
  22. Korean J Plant Tissue Culture v.24 Superoxide dismutase activity in suspension cultured cells of tomato (Lycopersicon esculentum Mill) You SH;Huh GH;Kwon SY;Lee HS;Bang JW;Kwak SS