Structural Characteristics of Vascular Tissue in Carrot Seedlings with Anomalous Cotyledon Developed from Somatic Embryos

당근의 체세포배로부터 발생한 이상자엽 유식물의 유관속 조직의 구조적 특성

  • 홍성식 (전남대학교 사범대학 생물교육과) ;
  • 소웅영 (전북대학교 자연과학대학 생물과학)
  • Published : 1998.01.01

Abstract

The somatic embryos of Daucus carota L. cv. Hongshim developed in MS basal medium far 4 weeks had varied number of cotyledons. Palisade and spongy parenchyma of cotyledon were not clearly discriminated in all seedlings developed from the embryos. No independent existence of collateral vascular bundle was observed in all seedlings with various types of cotyledon ; instead, vascular bundles were either interconnected or partially connected with one another. Most of the cotyledonary bases on hypocotyl showed short cylinder structure which encircle plumule. The vascular tissues of cotyledonary bases and nodes of seedlings with jar-shaped or 1 cotyledon were connected in ring forms, showing the pattern of ectophloic shiphonostele, and similar ring form structure was also found in the vascular arrangement of 5 cotyledon seedlings. The vascular bundles of seedlings with 2, 3 and 4 cotyledons in many cases had independently arranged within the cotyledonary bases and nodes, showing the pattern of eustele. In hypocotyl, tetrarch or hexarch xylems prevailed in seedlings with jar-shaped cotyledon or 1 and 5 cotyledon; tetrarch xylems prevailed in 2 cotyledon seedlings; and triarch xylems prevailed in 3 cotyledon seedlings. In most of seedlings, cortex vascular bundles were dispensed in the region from cotyledonary node to hypocotyl, but double vascular bundles were also observed occasionally. In roots, diarch xylems were observed in most of seedlings with 2 cotyledons, triarch xylems in half of seedlings with 3 cotyledons, and diarch xylems in most of the remaining seedlings with the occasional occurrences of tetrarch xylems.

당근(Daucus carota L cv. Hongshim)의 체세포배를 MS 기본배지에서 4주간 현탁배양하여 얻어진 유식물들은 자엽수에 따라 유형별로 구별되었고, paraffin method에 의한 영구표본을 제작하여 유관속 구조를 관찰하였다. 자엽의 엽육은 책상조직과 해면조직으로 뚜렷한 분화가 되어 있지 않았다. 모든 유형의 자엽에서 유관속은 독립된 병립유관속으로 배열되어 있지 않고, 서로 연결되어 있거나 부분적으로 연결되어 있다. 자엽기부는 대부분의 유형에서 짧은 원통상 구조를 보인다. 자엽기부와 자엽절에서 유관속의 배열은 주발형자엽 및 1자엽배에서는 환상으로 연결되므로서 외사관상중심주의 형태를 보이는 경우가 많고, 5자엽배에서도 가끔 관찰되었다. 2자엽, 3자엽 및 4자엽배의 자엽기부와 자엽절에서는 주로 독립적으로 배열된 진정중심주가 관찰되었다. 배축에서 유관속의 배열은 주발형자엽, 1자엽 및 5자엽배에서는 4-6원형 목부가, 2자엽배에서는 4원형 목부가 많고, 3자엽배에서는 3원형 목부가 많았다. 자엽절-배축에는 대부분의 경우에 피층유관속이 산재하며, 드물게 이중유관속도 나타난다. 2자엽배에서는 대부분이 2원형 목부이며, 3자엽배에서는 3원형 목부가 절반정도 관찰되며, 나머지에서도 주로 2원형 목부이지만 드물게 4원형 목부도 관찰되었다.

Keywords

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