목단피 추출물의 항균 및 항산화 작용

Antimicrobial and Antioxidative Activities from Moutan Cortex Extract

목단피의 메탄을 추출물과 용매 분획물을 이용하여 항균작용, 프리라디칼 소거작용 및 항산화 작용을 검색하고, HPLC와 GC를 이용하여 페놀화합물을 분석하였다. 항균작용은 에칠아세테이트 분획에서 가장 높게 나타났으며, 그람 음성균보다는 그람 양성균에 대하여 더욱 효과적이었다. 에칠아세테이트 분획의 MIC를 측정한 결과, 그람 양성균에 대하여는 156-1250$\mu\textrm{g}$/ml, 그람 음성균에는 2500-5000$\mu\textrm{g}$/ml 이었다. DPPH 라디칼에 대한 프리라디칼 소거활성과 linoleic acid를 이용한 항산화 시험에서도 에칠아세테이트 분획이 가장 높은 활성을 나타내었다. 에칠아세테이트 분획의 항산화 활성은 $\alpha$-tocopherol과 비슷하였다. 에칠아세테이트 분획으로부터 U와 HPLC를 이용하여 3종의 페놀화합물을 확인하였으며, 그 함량은 p-hydroxybenzoic acid 1.35%, methyl gallate 14.61%, gallic acid 4.01% 이었다.

Methanol extract and various solvent fractions from Moutan Cortex were tested for their antimicrobial activities, free radical scavenging activities and antioxidative activities, and phenolic compounds in ethylacetate fraction were analyzed by GC and HPLC. In the antimicrobial activities test, the ethylacetate fraction of methanol extract showed stronger than other fractions. The antimirobial activities were more effective against Gram positive bacteria than Gram negative bacteria. Minimum inhibitory concentration(MIC) of ethylacetate fraction showed 156-1250$\mu\textrm{g}$/ml against Cram positive bacteria and 2500-5000$\mu\textrm{g}$/mg against Gram negative bacteria. The free radical scavenging activities and antioxidative activities using linoleic acid were higher in ethylacetate fraction. The antioxidative activity of ethylacetate fraction was similar to ${\alpha}$-tocopherol. The 3 major phenolic compounds were analyzed by GC and HPLC and these content were determined. The content of p-hydroxybenzoic acid, methyl gallate and gallic acid were 1.35%, 14.61% and 4.01%, respectively.