NEW ANTI-AGING AND ANTI-WRINKLE COSMETIC INGREDIENT : INNER NUTSHELL OF CASTANEA MOLLISIMA BL (CHESTNUT)

  • Published : 1998.09.01

Abstract

Inner nutshell of Castanea mollisima BL (chestnut) has been used as an anti-aging and anti-wrinkle agent from the ancient time in east Asia. In order to develop new anti-aging and anti-wrinkle, ethanolic extract of inner nutshell of Castanea mollisima BL (Cor-285) was prepared and various biological activities were evaluated. Cor-285 showed potent antioxidant activity, Especially, Cor-285 possessed potent free radical scavenging activity in vitro (IC50:7.6 g/ml) compared to gallic acid (IC50:12.5 g/ml), Cor-285 showed the preventive effect against UV-induced cytotoxicity of fibroblast at concentration of 25-250 g/ml. When Cor-285 was evaluated for its anti-allergic activity, it effectively inhibited histamine release from mast cells induced by compound 48/80 (86% inhibition at 10 mg/ml). The inhibitory activity was stronger than that of glycyrrhiznate. Cor-285 also showed in vivo inhibition against delayed hypersensitivity as well as croton-oil induced ear edema in mice when topically applied These results strongly suggest that Cor-285 may reduce immunoregulatory 1 inflammatory skin trouble. From the attempts to isolate the constituents, citropten (simple coumarin) and ellagic acid, a well known radical scavenger, were isolated. In a clinical trial of twenty healthy volunteers with aged skin,6 weeks application of Cor-285 (3% cream) decreased wrinkle about 26% and increased moisturizing 20% on the skin. All of these results indicate that Cor-285 may be an effective anti-aging and anti-wrinkle agent.

Keywords

References

  1. Anal. Biochem. v.95 Ohakawa, T.;Ohishi, N.;Yagi, K.
  2. Yakugaku Zasshi v.108 Fugita, Y.;Uera, I.;Morimoto, Y.;Nakajima, M.;Hatano, C.;Okuda, T.
  3. Exp. Cell Res. v.18 Uvans, I. L.;Thon
  4. Japaness J. Allergy v.19 no.3 Hidehiko, H.;Ryouichi, E.;Yukimasa, O.
  5. Arch Pharm. Res. v.16 Kim, H. K.;Namgoong, S. Y.;Kim, H. P.
  6. Endocrinal. v.77 Tonneli, G.;Thiabalt, L.;Ringgler, I.
  7. J. Arzneim. Forsch./Drug Res. v.40 no.11 Tarayre, J. P.;Barbara, M.;Aliaga, M.;Tisneversailles
  8. Bioengineering of the skin. water and the stratum corneum Hardware and measuring principle : CORNEOMETER Courage, W.;Elsner P.(Ed.);Berardesca, E.(Ed.);Maibach H. I.(Ed.)
  9. Handbook of non-invasive methods and the skin Measurement of epidermal capacitance Barel, A.;Clarys, P.;Serup, J.(Ed.);Jemec, GBE(Ed.)
  10. Hydration Bioengineering of the skin : Methods and instrumentation Distante, F.;Berardesca, E.(Ed.);Elsner, P.(Ed.);Wilhelm, K. P.(Ed.);Maibach, H. I.(Ed.)
  11. Evaluation of moisturizers Bioengineering of the skin : Skin surface imaging and analysis Stab, F.;Sauermann, G.;Hoppe, U.;Wilhelm, K. P.(Ed.);Elsmer, P.(Ed.);Berardesca, E.(Ed.)Maibach, H. I.(Ed.)
  12. Skin elasticity Bioengineering of the skin : Methods and instrumentation Elsner, P.;Berardesca, E.(Ed.)Elsner, P.;Wilhelem, K. P.;Maribach, H. I.
  13. Cosmetics and toiletries manufacture world wide Comparative studies of skin roughness measurements by profilometry and a new iamge analysis system Nissen, H. P.
  14. Hand-book of non-invasive methods and the skin Stylus method for skin surface contour measurement Gassmueller, J.;Kecskes A.;Jahn, P.
  15. Oxidative damage and repair Davies, K. T. A.
  16. Mutation Res. v.250 Simic, M. G.;Bergtold, D. S.
  17. Environ. Mol. Mutagen. v.15 Hartman, P .E.;Shanke, D. M.
  18. Cancer Res. v.52 Wattenberg, L. W.
  19. Fragrance J. v.8 Active oxygen scavenging activity in plant extracts Masaki, H.
  20. Fragrance J. v.18 Reactive oxygen species scavenging effect of crude drug Fukuda, T.;Kitada, Y.
  21. Chem. Pharm. Bull. v.40 no.8 Yoshikawa, M.;Yamahara, J.