대한의용생체공학회:의공학회지 (Journal of Biomedical Engineering Research)

  • 제19권5호
  • /
  • Pages.441-448
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  • 1998
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  • 1229-0807(pISSN)
  • /
  • 2288-9396(eISSN)
대한의용생체공학회 (The Korean Society of Medical and Biological Engineering)
권호 표지

N-hydroxysuccinimidyl phenyl azide와 광반응을 이용한 펩타이드의 마이크로형태 고정화

Micropatterning of Peptides to Solid Surface by Deep-UV Lithography using N-hydroxysuccinimidyl phenol azide

  • 김진희 (바이오메드랩(주)) ;
  • 김현정 (바이오메드랩(주)) ;
  • 김종원 (바이오메드랩(주)) ;
  • 장준근 (서울대학교 정밀기계설계공동학연구소) ;
  • 민병구 (서울대학교 의학연구원 의용생체공학연구소) ;
  • 최태부 (건국대학교 공과대학 미생물공학과)
  • 발행 : 1998.10.01
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초록

폴리머 표면에 생체물질의 고정화 방법은 생체적합성을 자닌 재료의 개발에있어서 중요한 방법중의 하나이다. 광반응을 이용한 photolithography방법을 사용하여 재료 표면의 원하는 부위에 단백질을 고정할 수 있다. 본 연구에서는 파이브로넥틴의 세포부탁 리간드, GRGDS펩타이드를 N-hydroxysuccinimidyl phenol azide를 이용하여 미세한 선의 형태로 표면에 광반응으로써 고정하였다. 광반응 유도체가 고정된 표면은 형광물질을 사용하여 확인하였다. 또한 혈관내피 세포는 GRGDS펩타이드가 고정화된 표면에서만 부탁됨을 관찰하였다.

Defined spatial localization of biomolecules on the polymer surface Is potentially powerful method to generate biocompatible surface. Photolithography and photochemistry can be used to immobilize peptides only al a given region of the surface. In this study, peptide RGDS, one of the endothelial cells recognition sites of fibronectin, was covalently immobilized on the polystyrene coated surface with micropattern. It was performed by photochemical reactivity of a synthesized N-hydroxysuccinimidyl phenyl azide. The micropatterning was confirmed by staining with fluorescent dye, aminoacetamido fluorescein. Endothelial cell adhesion was observed only on the RGDS immobilized areas.

키워드

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