Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)
- Volume 18 Issue 1
- /
- Pages.69-76
- /
- 1998
- /
- 2287-5824(pISSN)
- /
- 2287-5832(eISSN)
Transformation of Brassica napus with Glutathione Reductase Gene
Glutathione reductase 유전자 도입에 의한 유채의 형질 전환
- Lee, Hyo-Shin (College of Agriculture, Kyungpook National University) ;
- Chung, Min-Sup (College of Agriculture, Kyungpook National University) ;
- Jo, Jin-Ki (College of Agriculture, Kyungpook National University)
- Published : 1998.03.30
Abstract
This study was conducted to construct of the transgenic plants wliich are resistant to oxidative stresses including ozone with B. mpestris cytosolic glutathione reductase cDNA using the binary vector system of Agrobacterium tumefaciens. The 1.8kb B. campestris cytosolic GR cDNA was subcloned into the unique Sma I site of the plant transformation vector pBKSI- I, downstream of the constitutive CaMV 35s promoter and upstream of the nos termination sequence, in place of the uidA (GUS) reporter gene. The resulting plant transformation vector, pBKS-GRI, was introduced into A. tumefaciens LBA4404 by two cycles of tkeze-thaw method. The B. nqus cotyledonary petioles were transformed by the Agrubaferium harboring pBKS-GRI. Transformed shoots were induced and selected on regeneration medium supplemented with kanarnycin. The shoot formation was increased remarkably by addition of Ag
Keywords