Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Comparison of Characteristics between Insecticidal and Noninsecticidal Basillus thuringiensis Strains belonging to Serotype H8a8b

  • Roh, Jong-Yul (college of Agriculture and Life Sciences, Seoul National University, Suwon) ;
  • Je, Yeon-Ho (college of Agriculture and Life Sciences, Seoul National University, Suwon) ;
  • Park, Hyun-Woo (Department of Entomology, University of California Riverside, USA) ;
  • Chang, Jin-Hee (College of Agriculture and Life Sciences, Seoul National University, Suwon) ;
  • Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Dae-Weon (college of Agriculture and Life Sciences, Seoul National University, Suwon) ;
  • Ziwen Yang (college of Agriculture and Life Sciences, Seoul National University, Suwon) ;
  • Kang, Seok-Kwon (college of Agriculture and Life Sciences, Seoul National University, Suwon)
  • Published : 1998.12.01
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Abstract

A noninsecticidal strain, Bacillus thuringiensis NTB-88, isolated from Korean soil, had a typical bipyramidal parasporal inclusion and its serotype is identical to B. thuringiensis subspmorrisoni (H8a8b). To elucidate differences between insecticidal and noninsecticidal strains, we compared strain NTB-88 to other toxic B. thuringiensis subsp. morrisoni strains (HD-12 and PG-14). Restriction endonucleases digested plasmid DNA patterns showed that strain NTB-88 was different from lepidopteran-toxic strain, HD-12, but it was similar to dipteran-toxic strain, PG-14. The gene type of strain NTB-88 was different from those of other insecticidal strains, Furthermore, the NH2-terminal amino acid sequence of crystal protein of strain NTB-88 had no relation to those of the previously known $\delta$-endotoxins in other toxic strains as well as HD-12 and PG-14 strains. Therefore, the noninsecticidal crystal protein in strain NTB-88 is novel and its property is different from insecticidal ones.

Keywords

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