Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
권호 표지

Biochemical Properties of Haemolymph Carboxylesterase in Diapausing Pupae of Helicoverpa assulta (Guenee)

담배나방의 휴면 용 혈림프 Carboxylesterase의 생화학적 특성

  • 김영관 (한남대학교 이과대학 생물학과) ;
  • 이형철 (한남대학교 이과대학 생물학과) ;
  • 박희윤 (한남대학교 이과대학 생물학과) ;
  • 이옥경 (한남대학교 이과대학 생물학과) ;
  • 유종명 (한남대학교 이과대학 생물학과)
  • Published : 1998.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Haemolyph carboxylesterases induced in diapausing pupae of Helicoverpa assulta Guenee were investigated. Increase in the activity of the electrophoresed isozyme bands were observed during the diapausing pupae. The isozymatic composition exhibited remarkable alterations represented as disappearance and induction of some isozyme bandsp which were identified as carboxylesterase (CE) on the basis of their specificities to inhibitors. Much higher activity of the induced CE was shown in reaction with $\beta$-naphthyl acetate ($\beta$-Na) than $\alpha$-naphthyl butyrate ($\alpha$-Nb), representing the high regioselectivity to $\beta$-naphthyl group. Optimal temperature for the enzyme activity was different to the substrates used 37$^{\circ}C$ in $\beta$-Na and 4$0^{\circ}C$ in $\alpha$-Nb, respectively. However, the optimal pH for the enzyme activity was the same as 7.5 regardless of the substrates used, and relatively high thermostability of the CE was demonstrated by showing the denaturation at high temperature (50~55$^{\circ}C$).

Keywords

References

  1. Insect Biochem v.6 Larval and adult housefly carboxylesterase; Isozymatic composition and tissue pattern Ahmad, S.
  2. Drug, Metab. Rev. v.5 Nonoxidative enzymes in the metabolism of insecticides Ahmad, S.;A. J. Forgash
  3. Korean J. Appl. Entomol v.29 Initiation and termination of pupal diapause in the oriental toabacco budworm(Heliothis assuilta) Boo, K. S.;H. C. Shinn;M. W. Han;M. H. Lee
  4. Appl. Ent. Zol v.2 Comparative studies on esterase zymograms among various tissues in the silk worm, Bombyx mori L.(Lepidoptera:Bombycidae) Eguchi, M.;N. Yoshitake
  5. J. Histochem. Cytochem v.16 Nonspecific esterases in mosquitos Freyvogel, T. A.;R. L. Hunter;E. M. Smith
  6. Comp. Biochem. Physiol v.49B The distribution of acetylcholine and unspecific esterases in the midgut of female Aedes aegypti L Geering, K.;T. A. Freyvogel
  7. Appl. Entomol. Zool v.18 Electophoretic esterase patterns in the brown planthopper Nilaparavarta lugens Stal.(Hemiptera: Delphacidae) shich developed resistance to insecticides Hideaki, H.;K. Ozaki
  8. J. Insect Physiol v.29 Photoperiodic and thermal regulation of antifreeze protein levels in the beetle Dendroides canadensis Horwath, K. L.;J. G. Duman
  9. Insect Biochem v.10 Esterases in larval tissues of gypsy moth, Lymantria dispar(L.); Optimum assay conditions, quantification and characterization Kapin, M. A.;S. Ahmad
  10. Biochem. Genet v.4 A profile of Drosophila species enzymes assayed by electrophoresis-I. Number of alleles, heterozygosites, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes Kojima, K.;J. Gillespie;Y. N. Tobari
  11. Nature v.227 Clevage of structural proteins during assembly of the head of bacteriophage $T_4$ Laemmli, U. K.
  12. Insect Biochem v.17 Characterization if acetylcholinesterase activity in the larval brain of Monduca sexta Lester D. S.;L. I. Gilbert
  13. Biochem J. v.54 The hydrolysis of esters of phenol by cholinesterases and other esterases Mounter, L. A.;V. P. Whittaker
  14. Appl. Ent. Zool v.29 Some properties of carboxyl esterase from Aphis gossypii Glover (Homoptera: Aphidiadae) Owusu, E. O.;K. Komi;M. Horiike;C. Hirano
  15. Appl. Entomol. Zool v.9 Naphthyl acetate esterase in the green rice leafhopper, Nephotettix cinticeps Uhler, with special reference to the resistant colony of the organophosphorus insecticide Ozaki, K.;H. Kokie
  16. Insect Biochem. Physiol v.31 Biochemical characterization and purification of esterases from three strains of german cockroach, Blattell germanica(Dicyoptera: Blattellidae) Prabhakaran, S. K.;S. T. Kamble
  17. Can. Ent v.106 Enzyme polymorphism in the cornearworm, Heliothis zea (Lepidoptera: Noctuidae). Haemolymph esterase polymorphism Sell, D. K.;G. S. Whitt;R. L. Metcalf;L. P. Lee
  18. Insect Biochem v.9 Studies of esteras 6 in Drosophila melanogaster. Ⅲ. The developmental pattern and tissue distribution Sheehan, K.;R. C. Richmond;B. J. Coohrane
  19. Pest. Art. News. Summ v.19 Sime hydrolase and their involvement in insecticide resistance Sudderudin, K. I.;H. H. Tan
  20. Appl. Ent. Zool v.14 Enzyme associated with glycogen metabolism in larvae of the rice stem borer Chlio suppressalis Walker: Some properties and changes in activities during hibernation Tsumuki, H.;K. Kanehisa
  21. Appl. Ent. Zool v.18 Electrophoretic analysis of nonspecific esterases and acetylcholinesterases from the housefly, Musca domestica L.(Diptera: Muscidae), with reference to organophosphorus insecticide resistance Ugaki, M.;T. Abe;J. Fukami;Shono
  22. Isozyme ontogeny of the mosquito, Anophepheles albimanus. In Isozymes: Ⅲ. Developmental biology Markert Vedbrat S. S.;G. S. Whitt;C. L. (ed.)
  23. Proc. Nalt. Sci. U. S. A. v.69 Juvenile hormone induction of esterases: A mechanism for the regulation of juvenile hormone titre Whitmore,D.;Jr.;E. Whitmore;L. I. Gilbert
  24. Insect Biochem. Mol. Biol v.24 Isolation of an esterase conferring insecticide resistance in the mosquito Culex tarsalis Whyard, S. A.;E. R. Downe;V. K. Walker
  25. Korean J. Ento v.11 Nonspecific esterase patterns of camphor silk moth Dictyoploca japonoca Moore Yoo, C. M.
  26. Korean J. Zool v.39 Substrate and inhibitor specificities of esterase in Lucilia illustris Meign Yoo, C. M.;C. B. Bak;H. C. Lee
  27. J. Sci. Res. Han Nam Univ v.26 Identification and purification of cold stress protein(CSP) in Helicoverpa assulta Yoo, C. M.;E. D. Yoo;S. H. Kim;H. C. Lee
  28. Kor. J. Entomol v.21 The changes of organic compounds and the related enzymes of overwintering larva in the pine needle gall midge, Thecodiplosis japonensis Yoo, C. M.;S. E. Jeong;H. R. Kim
  29. J. Econ. Entmol v.83 Properties of esterases from Lygus esperus Knight(Hemiptera : Miridae) and the roles of the esterases in insecticide resistance Zhu, K. Y.;W. A. Brindley