Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
권호 표지

Induction of Ovulation by LHRH-a and Pimozide in the Bullhead, Pseudobagrus fulvidraco

LHRH-a 와 pimozide에 의한 동자개의 배란유도

  • 박홍양 (건국대학교 동물자원연구센타) ;
  • 이종영 (건국대학교 동물자원연구센터) ;
  • 이영직 (해양수산부 어업제도과) ;
  • 권혁추 (선문대학교 식량자원학부)
  • Published : 1998.05.01
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Abstract

Effects of intraperitoneal administration of LHRH-a (luteinizing hormone releasing hormone analogue), pimozide and HCG (human chorionic gonadortropin) were studied on ovulation in the bullhead, Pseudobagrus. fulvidraco. Ovulatin was induced in 71.4, 80 and 100% by injection of HCG 5,000, 10,000 and 20,000 IU/kg of body weight, respectively. Also, the majority of fish injected with LHRH-a in the presence or absence of pimozide were ovulated. The ovulating rates in fish injected with LHRH-a alone showed 50, 75 and 100% at a dose 200, 300 and $400^{\mu}$g/kg, respectively. Pimozide enhanced the activity of LHRH-a at $300^{\mu}$g/kg plus pimozide at 1 mg/kg. Pimozide alone was ineffective in inducing ovulation of P. fulvidraco. There were no significant differences in gonadosomatic index (GSI) and pseudo-GSI values among the groups treated with HCG, LHRH-a and LHRH-a plus pimozide (P>0.05). The latency time groups treated with HCG was 17~22 hours, and the group injected with LHRH-a alone and/or in combination with pimozide was ovulated within 23~29 hours.

동자개의 배란유도에 대한 LHRH-a, pimozide 및 HCG의 복강내 투여효과에 대해 조사하였다. 어체중 kg당 HCG를 각각 5,000, 10,0000, 20,000 IU를 주사하였을 경우 71.4, 80, 100%의 배란유도율을 나타냈다. 또한 pimozide의 존재에 관계없이 LHRH-a를 주사한 개체들 대부분이 배란되었다. LHRH-a 단독투여 실험에서 kg당 200, 200, $400^{\mu}$g 처리에서 각각 50, 75 및 100%의 배란유도율을 나타냈다. pimozide첨가는LHRH-a의 작용을 향상시켰다. 즉, LHRH-a와 pimozide의 혼합투여에서 어체중 kg당 pimozide 1 mg과 LHRH-a $300^{\mu}$g에서 100%의 가장 좋은 배란유도율을 나타내었다. 그러나 pimozide 단독투여에서 배란이 유도되지 않았다. 호르몬 처리후의 생식소숙도지수(GSI)와 pesudo-GSI의 변화를 조사한 결과, HCG, LHRH-a 및 LHRH-a+pimozide를 주사한 그룹간의 생식소숙도지수(GSI)와 pseudo-GSI에 있어서 퉁계적인 유의차는 없었다(P>0.05). 호르모처리후 배란이 일어나기까지의 소요시간은 HCG처리후 17~22시간, LHRH-a단독 또는 pimozide와의 혼합처리에서 대체로 23~29시간이 걸렸다.

Keywords

References

  1. Bull. Jpn. Soc. Sci. Fish. v.44 Induced of ovulation in place and goby with synthetic LH releasing hormone Aida, K.;R. Z. Iznmo;H. Satah;T. Hibiya
  2. Aquaculture v.41 Effects of LHRH and LHRH-A on plasma GtH levels and maturation/ovulation in the common carp, Cyprinus carpio, kept under various environmental conditions Billard, R. K.;Bienarz, R.E.;Peter, M.;Sokolowska, C. Weil;L.W. Crim
  3. Aquaculture v.62 Stimulation of gonadotropin secretion and spermiation in carp by pimozide-LHRH-a treatment : Effects of dose and time of day Billard, R.;K. Bieniarz;W. Popek;P. Epler;B. Breton;K. Alagarswami
  4. Aquaculture v.35 Accelerated ovulation by pelleted LHRH-analogue treatment of spring-spawning rainbow trout held at low temperature Crim, L.W.;A.M. Sutterlin;D.M. Evans;C. Weil
  5. Aquaculture v.43 Advancement and synchrony of ovulation in Atlantic salmon with pelleted LHRH analogue Crim, L.W.;B.D. Glebe
  6. Fish physiology v.IX no.B Induced final maturation, ovulation and spermiation in cultured fish Donaldson, E.M.;G.A. Hunter;Hoar, W.S.(ed.);D. Randall(ed.);E.M. Donaldson(ed.)
  7. Aquaculture v.119 Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH superactive analogue combined with metoclopramide : analysis of hormone profile, progress of oocyte maturation and dependence on temperature Droni, S.;M. Ofir;B. Levavi-Sivan;Z. Yaron
  8. Biometrics v.11 Multiple range and multiple tests Duncan, D.B.
  9. Aquaculture v.93 LHRH-a and domperidone-induced oocyte maturation and ovulation in bighead carp, Aristichthys nobilis (Richardson) Fermin, A.C.
  10. Aquaculture v.86 Advancement of sexual maturation and spawning of sea bass, Lates calcarifer (Bloch), using pelleted luteinizing hormone-releasing hormone analogue and $17^{\alpha}$-methyltestosterone Garcia, L.;Ma. B.
  11. Aquaculture v.95 Effect of a synthetic LHRH analogue of bream, Abramis brama L Glubokov, A.I.;N.N. Motloch;M. A. Sedova
  12. Aquaculture v.97 Induced spawning of Indian major carps and maturation of a perch and a catfish by murrel gonadotropin releasing hormone, pimozide and calcium Halder, S.;S. Sen;S. Ghattacharya;A.K. Ray;A. Ghosh;A.G. Jhingran
  13. Aquaculture v.47 Induced spawning of sea bass, Labeo calcarifer, and rabbit fish, Siganu guttaus, after implantation of pelleted LHRH analogue Harvey, B.;J. Nacario;L.W. Crim;J.V. Juriano;C.L. Marte
  14. Nippon Suisan Gakkaishi v.40 Induction of ovulation in the ayu with LH-releasing hormone (LH-RH) Hirose, K.;R. Ishida
  15. Aquaculture v.104 Gonadotropic hormone-releasing hormone analog (GnRH-A) induced ovulation and spawning in female winter flounder, Pseudopleuronectes americanus (Walbaum) Harmin, S.A.;L.W. Crim
  16. The 2nd Japan-Korea joint meeting and symposium on aquaculture Induced ovulation and spawning by hormone in fish Kwon, H.C.
  17. Can. J. Fish Aquat. Sci. v.39 Applications of endocrinology to fish culture Lam, T.J.
  18. Aquaculture v.62 Induced spawning of grey mullet (Mugil cephalus) by LHRH-a Lee, C.S.;C.S. Tamaru;G.T. Miyamoto;C.D. Kelley
  19. Aquaculture v.95 Effects of gonadotropin-releasing hormone agonists and dopamine antagonists on gonadotropin secretion and ovulation in Chinese loach, Paramisgurnvs dabryanus Lin, H.R.;X.J. Zhoh;G.V.D. Kraak;R.E. Peter
  20. Aquaculture v.60 Induced spawning of maturing milkfish (Chanos chanos Forsskal) with gonadotropin-releasing hormone (GnRH) analogues administered in various ways Marte, C.L.;N.M. Sherwood;L.W. Crim;Harvey
  21. Aquaculture v.102 Advancement and synchronization of ovulation in Atlantic salmon (Salmo salar L.) following injections of LHRH analogue Taranger, G.L.;S.O. Stefansson;T. Hansen
  22. Aquaculture v.80 Dietary administration of an LHRH analogue induces spawning of spotted sea trout (Cynoscion nebulosus) Thomas, P.;N.W. Boyd
  23. Gen. Com. Endocrinol. v.49 Effects of LHRH and des-$Gly^{10}(D-Ala^6)$ LH-RH-ethylamide on plasma gonadotropin levels and oocyte maturation in adult female coho salmon (Oncorhynchus kisutch) Van der Kraak, G.;H.R. Lin;E.M. Donaldson;H.M. Dye;G.A. Hunter
  24. 한국양식학회 추계학술발표대회 요지 LHRH-a 와 pimozide에 의한 한국산 메기의 배란유도 권혁추;김용득;이영직;박흥양
  25. 한국양식학회지 v.9 no.3 생식소자극호르몬 방출호르몬 유사물질에 의한 메기(Silurus asotus)의 배란유도 권혁추;최낙중;박흥양
  26. 한국수산학회지 v.30 no.2 동자개(Pseudobagrus fulvidraco)의 난모세포 성숙과 배란에 대한 스테로이드와 HCG의 in vitro 효과 임상구;백혜자;한창희
  27. 한국어도보 정문기