Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Activities of Phospholipase $A_2$ and Cyclooxygenase, and Syntheses of Thromboxane and Prostacyclin in Streptozotocin Induced Diabetic Rats

Streptozotocin 유도 당뇨쥐에서의 Phospholipase $A_2$, Cyclooxygenase 활성과 Thromboxane 및 Prostacyclin합성

  • 이순재 (대구효성가톨릭대학교 식품영양학과) ;
  • 양정아 (대구효성가톨릭대학교 식품영양학과) ;
  • 김성옥 (대구효성가톨릭대학교 식품영양학과) ;
  • 최정화 (대구효성가톨릭대학교 식품영양학과) ;
  • 곽오계 (대구전문대학 간호과) ;
  • 장현욱 (영남대학교 약학대학)
  • Published : 1998.02.01
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Abstract

The relation between lipid peroxidation and thrombotic reaction were investigated in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100$\pm$10gm were randomly assigned to normal and STZ-induced diabetic group(DM). Diabetes was experimentally induced by intravenous injection of 55mg/kg of body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of basal diet. Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in diabetic group after STZ injection. Serum levels of thiobarbituric acid reacting substances(TBARS) that were markedly increased in DM group compared with of normal group. TBARS levels of HDL and LDL were similar patterns to total TBARA of serum. Activities of platelet phospholipase A2(PLA2) were higher in diabetic group than those of normal group. Activities of platelet cyclooxygenase were 106% in DM group than normal group. Platelet thromboxane A2(TXA2) formation was increased in DM group than normal group. Production of aortic prostacyclin(PGI2) was lower in diabetic group than that of normal group. PGI2/TXA2 ratios were decreased by 55% in DM groups than those of normal group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stess which leads to acceleration of lipid peroxidation and platelet aggregability. In conclusion, accelerating effect of lipid peroxidation and thrombogenesis in diabetic state is regareded to be resulted from enhancement of PLA2 activity and arachidonic acid metabolism, inhibition of antiaggrgating agent and aortic PGI2 formation.

Keywords

References

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