Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

The Effect of Ascorbic Acid on the Enzyme Reaction in Pyridinoline Formation during Soluble Collagen Maturation

비타민 C가 가용성 콜라겐의 성숙과정에서 Pyridinoline 생성 효소계에 미치는 영향

  • Published : 1998.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Normal tensile strength in collagen fibrils is due to intermolecular and intramolecular crosslinks which are known to be altered in aging. Pyridinoline, a mature crosslink which is stable and nonreducible, is derived from two hydroxyallysine and one hydroxylysine residues of collagen fibrils. The excess formation of pyridinoline in collagen is associated with making the tissue stiffer, less soluble and less digestible by enzymes. Lysyl oxidase is the enzyme that initiates the biosynthesis or crosslinks in collagen by catalyzing the oxidative deamination of the lysyl and hydroxylysyl residues in these molecules, and its activity is inhibited by $\beta$-aminopropionitrile(BAPN). Our previous work demonstrated that the pyridinoline content of bone collagen significantly was increased during incubation for 5 weeks at 37$^{\circ}C$ invitro, but it was diecrased by the addition of ascorbic acdi(AsA). In this study, we clarified the specific action of AsA in aging process in vitro enzymatic reaction.

Keywords

References

  1. 現代化學 v.8 コラ-ゲン分子の變化とエシング-老化をくいとめる方法みつかるか 藤本大三郞
  2. Biochem. Biophys. Res. Commun. v.109 Aging and cross-linking in human aorta Fujimoto, D.
  3. Biochem. J. v.163 The chemistry of the collagen cross-links Simon, P. R.;Allen, J. B.
  4. Collagen in Health and Disease Turnover and cross-linking of collagen Simon, P. R.
  5. Biochem. Biophys. Res. Commun. v.152 Aging and cross-linking of skin collagen Yamauchi, M.;Woodley, D. T.;Mechanic, G. L.
  6. Calcif. Tissue Int. v.34 Comparative molecular distribution of cross-links in bone and dentin collagen Kuboki, Y.;Mechanic, G. L.
  7. J. Biologic. Chem. v.252 Collagen cross-linking effect of D- penicillamine on cross-linking in vitro Siegel, R. C.
  8. Biochem. J. v.252 collagen cross-linking in human bone and articular cartilage Eyre, D. R.;Dickson, I. R.;Ness, K. V.
  9. J. Nutr. Sci. Vitaminol. v.40 Age-related changes in the pyridinoline content of guinea pigs cartilage and achilles tendon collagen Kim, M.;Otsuka, M.;Arakawa, N.
  10. J. Biochem. v.83 Pyridinoline, a nonreducible crosslink of collagen Fujimoto, D.;Moriguchi, T.
  11. Biochem. Biophys. Res. Commun. v.84 The structure of pyridinoline, a collagen crosslink Fujimoto, D.;Moriguchi, T.;Hayashi, H.
  12. Biochem. Biophys. Res. Commun. v.109 The structures of pyridinoline Barber, M.;Bordoli, R.;Elliott, G. J.;Fujimoto, D.;Scott, S. E.
  13. Biochem. Biophys. Res. Commun. v.104 Pyridinoline is a real moiety of collagen Tsuda, M.;Ono, T.;Ogawa, T.;Kawamishi, Y.
  14. Proc. Natl. Acad. Sci. USA v.61 The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to α-aminoadipic-δ-semialdehyde(allysine) by an extract from bone Pinnell, S. R.;Martin, G. R.
  15. Arch. Biochem. Biophys. Arch. Biochem. Biophys. v.149 The production of lysyl oxidase by human fibroblasts in culture Layman, D. L.;Narayanan, A. S.;Martin, G. R.
  16. Arch. Biochem. Biophys. v.162 Stability and purification of lysyl oxidase Narayanan, A. S.;Siegel, R. C.;Martin, G. R.
  17. Cinica. Chimica. Acta v.71 Lysyl oxidase activity in human normal skins and postburn scars Hayakawa, T.;Hino, M.;Fuyamada, H.;Nagatsu, T.;Aoyama, H.;Izawa, Y.
  18. J. Biol. Chem. v.251 Collagen cross-linking Siegel, R. C.;Fu, J. C. C.
  19. Biochem. J. v.177 Puridication and properties of four species of lysyl oxidase from bovine aorta Kagan, H. M.;Sullivan, K. A.;Olsson, T. A.;Cronlund, A. L.
  20. Biochem. Biophys. Res. Commun. v.108 Collagen cross-linking : Lysyl oxidase dependent synthesis of pyridinoline in vitro: confirmation that pyridinoline is derived from collagen Siegel, R. C.;Fu, J. C. C.;Uto, N.;Horiuchi, K.;Fujimoto, D.
  21. 한국식품영양과학회지 v.26 In vitro aging에 있어서 콜라겐 성숙가교의 변화에 대한 비타민 C의 영향 김미향
  22. J. Biochem. v.83 Pyridinoline, a nonreducible crosslink of collagen Fujimoto, D.;Moriguchi, T.
  23. 酵素科學シンポジウム v.16 2,3-Diketo-L-gulonic acidの合成方法 香川靖雄;龍口久
  24. J. Biol. Chem. v.193 protein measurement with the Folin phenol reagent Lowry, O. H.;rosebrough, N. J.;Farr, A. L.;Randall, R. J.
  25. J. Nutr. Sci. Vitaminol. v.38 An improved high performance liquid chromatographic assay for the determination of pyridinoline in connective tissues Arakawa, N.;Kim, M. H.;Otsuka, M.
  26. Arch. Biochem. Biophys. v.93 the determination of hydroxyproline in tissue and protein samples containig small proprotions of this imino acid Woessner, J. F.
  27. Inter. Rew. Connec. Tissue Res. v.8 Lysyl oxidase Siegel, R. C.
  28. Biochem. J. v.167 Evidence for the role of vitamin $B_6$ as a cofactor of lysyl oxidase Murray, J. C.;Levene, C. I.
  29. Biochem. Biophys. Res. Commun. v.108 Lysyl oxidase: evidence that pyridoxal phosphate is a cofactor Bird, T. A.;Levene, C. I.
  30. Adv. Exp. Med. Biol. v.48 Purification and properties of lung lysyl oxidase, a copper-enzyme Shieh, J. J.;Yasunobu, K. T.
  31. 生體と金屬イオン 高分子錯體硏究會(編)
  32. 水溶性ビタミン 日本學會(編)
  33. コラ-ゲン代射と疾患 永井裕;藤本大三郞
  34. Vitamin C: its molecular biology and medical potential Lewin, S.