Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Characteristics of Caseinomacropeptid(CMP) Purified from Whole Casein by Using Immobilized Chymosin

고정화 Chymosin을 이용하여 Whole Casein으로부터 분리한 Caseinomacropeptide(CMP)의 특성

  • 장해동 (한남대학교 식품영양학과) ;
  • 김의수 (한남대학교 식품영양학과)
  • Published : 1998.12.01
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Abstract

Chymosin was purified from commercial rennet with DEAE Sepharose CL 6B and immobilized on CeliteTM using glutaraldehyde. Whole casein from fresh raw milk was hydrolyzed by immobilized chymosin and total CMP was isolated by trichloroacetic acid(TCA) and ultrafiltration, and characterized. The amount of chymosin purified from 15g commercial rennet by DEAE Sepharose CL 6B was 0.16g and 18mg of chymosin was immobilized on 1g of CeliteTM by 5% glutaraldehyde. Immobilized chy mosin hydrolyzed most of casein on whole casein within 2 hours to leave para casein and casei nomacropeptide(CMP). The total CMP isolated from 10g of whole casein hydrolyzate by TCA and ultrafiltration was 0.4g and 0.1g, respectively. Results of electrophoresis, amount of sialic acid, com position of amino acid and ratio of A280 to A214 showed that total CMP by TCA was purer and had more CMP without carbohydrate than one by ultrafiltration. CMP isolated from total CMP by 12% TCA precipitation was 50% of total CMP and most of caseinoglycopeptide(CGP) was removed from total CMP, indicating less amount of sialic acid in CMP than in total CMP.

Keywords

References

  1. Milchwissenschaft v.48 pH-dependent molecular weight changes of k-casein glycomacropeptide and its preparation by ultrafiltration Kawasaki, Y.;Kawakami, H.;Tanimoto, N.;Dosako, S.;Tomizawa, A.;Kotake, M.;Nakajima, I.
  2. Int. Dairy J. v.6 Characteristic and potential uses of the caseinomacropeptide Abd El-Salam, M. H.;El-shibiny, S.;Buchheim, W.
  3. Fiziol. Zh. SSSR Im. I. M. Sechenova v.72 Study of the k-casein peptide action on rat gastric secretion by using atomatic quantitative recording of acid production Aleink, S. I.
  4. Fiziol. Zh. SSSR Im. I. M. Sechenoval v.72 Study of the mechanism of acid secretion inhibition and k-casein peptides in the stomach Aleink, S. I.;Stan, C. Y.;Cherinkov, M. P.
  5. Bull. Eksp. Biol. Med. v.102 Isolation, amino acid composition and biological effect of a peptide bioregulator from bovine k-casein Stan, C. Y.;Zhuravlev, B. V.
  6. Vopr. Med. Khim. v.33 Peptidic bioregulator from cow k-casein Stan, C. Y.;Elkimovskii, A. P.
  7. Eur. Pat. Appl. EP. v.283 Caseinoglycopeptides as dental plaque and dental caries inhibiting agents Neeser, J. R.
  8. Infect. Immunity v.56 Specific and nonspecific inhibition of adhesion of oral actinomyces and streptococci to erythrocytes and polystyrene by caseinoglycopeptide derivatives Neeser, J. R.;Chambez, A.;Delvedova, S.;Prigent, M. I.;Guggenheim, B.
  9. J. Agric. Biol. Chem. v.48 Bifidus growth-promoting activity of a glycomacropeptide derived from human k-casein Azuma, N.;Yamauch, K.;Mitsuoka, T.
  10. J. Agric. Food Chem. v.39 Bovine milk k-casein trypsin digest is a growth enhancer for the genus Bifidobacterium Poch, M.;Bezkorovainy, A.
  11. Milchwissenschaft v.47 Determination of k-casein glycomacropeptide by high performance liquid chromatography without trichloroacetic acid pretreatment Kawakami, H.;Kawasaki, Y.;Dosako, S.;Tanimoto, M.;Vakajima, I.
  12. Igaku to Seibutsugaku v.115 Effect of bovine k-casein on the growth of lactic acid bacteria Ito, O.;Kamato, S.;Yamano, W.;Yokojama, K.
  13. Milchwissenschaft v.26 Properties of basic glycopeptides released from cow milk protein by heat Lahav, E.;Edelsten, D.;Sode-Mogensen, M. T.;Safer, E.
  14. Le Lait v.73 Growth promoting activity of tryptic digest of caseinomacropeptide for Lactococcus lactic ssp. lactis Bouhallab, S.;Favrot, C.;Maubois, J. L.
  15. Biochem. J. v.271 Liberation of tryptic fragments from caseinomacropeptide of bovine k-casein involved in platelet function Leonil, J.;Molle, D.
  16. Biotechnol. Letters v.14 Tryptic hydrolysis of caseinomacropeptide in membrane reactor: preparation of bioactive peptides Bouhallab, S.;Molle, D.;Leonil, J.
  17. Eur. J. Biochem. v.158 Analogy between fibrinogen and casein Jolles, P.;Levy-Toledano, S.;Fiat, A.-M.;Soria, C.;Gillessen, D.;Thomaidis, A.;Dunn, F. W.;Caen, J. P.
  18. Le Lait v.71 Le peptides du lait a activite physiologique. Ⅲ peptides de lait a effect cardiovascullaire: activites antithrombotique et antihypertensive Maubois, J. L.;L'eonil, J.;Trove, R.;Bouhallab, S.
  19. French Pat. Appl. FR 2646775 Al. Use of the caseinoglycopeptide particularly from cow milk in the manufacture of a composition and particularly of a medicine for use in the prevention and treatment of thrombosis Droust, L.;Solliear, C.;Mazoyer, E.;Levy-Toledano, S.;Jolles, P.;Fiat, A. M.
  20. J. Dairy Sci. v.74 A new isolation method of caseinomacropeptide from sweet cheese whey Saito, T.;Yamaji, A.;Itoh, T.
  21. J. Food Sci. v.53 Fractionation and Characterization of glycomacropeptide from caseinate and skim milk hydrolysates Morr, C. V.;Seo, A.
  22. J. Dairy Sci. v.75 Proteolytic activity of proteinases on macropeptide isolated from k-casein Schammet, K. M.;Brown, R. J.;McMahon, D. J.
  23. Milchwissenschaft v.47 Characteristics of macropeptide fraction isolated from whole casein and purified k-casein Schammet, K. M.;McMahon, D. J.;Brown, R. J.
  24. Biosci. Biotech. Biochem. v.56 Large-scale preparation of k-casein glycomacropeptide from rennet casein whey Tanimoto, M.;Kawasaki, Y.;Dasako, S.;Ahiko, K.;Nakajima, I.
  25. Milchwissenschaft v.51 Isolation of glycomacropeptide from sodium caseinate hydrolyzate solution by ultrafiltration Chu, L.;Macleod, A.;Ozimek, L.
  26. J. Dairy Sci. v.40 Heterogeneity of crystalline rennin Ernstrom, C. A.
  27. Cheju National University J. v.8 Degradation of bovine para-k-casein by the action of chymosin Yoon, C. H.
  28. J. Dairy Sci. v.58 Preparation of an active form of immobilized rennin Brown, R. J.;Swaisgood, H. E.
  29. J. Dairy Sci. v.52 Discontinuous gel electrophoresis of whey proteins, casein and clotting enzymes Melachouris, N.
  30. J. Applied Biochem. v.6 Preparation and characterization of thionyl chloride activated succinamidopropyl glass as a covalent immobilization matrix Duval, G.;Swaisgood, H. E.;Horton, H. R.
  31. Methods in Enzymology v.44 Immobilized enzymes Mosbach, K.
  32. J. Dairy Sci. v.66 Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins Church, F. C.;Swaisgood, H. E.;Porter, D. H.;Catignani, G. L.
  33. J. Dairy Sci. v.74 Use of the o-phthalaldehyde and N-acetyl-L-cysteine reagent in the evaluation of milk proteins Hernandez, M. J. M.;Domingo, E. B.;Camanas, R. M. V.;Alrarez-Coque, M. C. G.
  34. J. Biol. Chem. v.234 The thiobarbituric acid assay of sialic acids Warren, L.
  35. 한국낙농학회지 v.8 Mucor SPP L42 응유효소의 고정화에 관한 연구 박종대;이준엽;고영숙
  36. Biotech. Bioeng. v.16 Immobilized tannase Weetall, H. H.;Detar, C. C.