Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Elimination of Spore Bacteria in Beef by Gamma Irradiation

감마선 조사에 의한 우육오염 포자세균의 제거

  • Kim, Sung (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Yook, Hong-Sun (Dept. of Food Irradiation, Korea Atomic Energy Research Institute) ;
  • Choi, Cheong (Dept. of Food Science and Technology, Yeungnam University) ;
  • Kim, Jeung-Ok (Department of Home Economics, King Sejong University) ;
  • Byun, Myung-Woo (Dept. of Food Irradiation, Korea Atomic Energy Research Institute)
  • 김성 (한국원자력연구소 방사선식품공학연구실) ;
  • 육홍선 (한국원자력연구소 방사선식품공학연구실) ;
  • 최청 (영남대학교 식품가공학과) ;
  • 김정옥 (세종대학교 가정학과) ;
  • 변명우 (한국원자력연구소 방사선식품공학연구실)
  • Published : 1998.09.01
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Abstract

The effect of gamma irradiation on the survival of spore bacteria was investigated in frozen cells ($-18^{\circ}C$) with 0.1 M phosphate buffer and inoculated cells in beef. In the case of the frozen cells at log phase, the radiation $D_{10}\;and\;12D_{10}$ values were 0.29 kGy and 3.48 kGy in Bacillus subtilis, 0.39 kGy and 4.68 kGy in Bacillus cereus and 0.46 kGy and 5.52 kGy in Clostridium perfrigens. And inactivation factors were 6.52~10.34 and 10.87~17.24 at the dosage of 3 kGy and 5 kGy, respectively. The radiosensitivity of inoculated cells in beef showed the $D_{10}$ value of 0.59~0.76 kGy, the $12D_{10}$ value of 7.08~9.12 kGy, and inactivation factors of 3.95~8.47. The radiosensitivity of the frozen cells was higher than that of inoculated cells in beef.

포자형성 세균인 Bacillus subtilis, Bacillus cereus 및 Clostridium perfrigens의 $-18^{\circ}C$동결균체와 우육에 접종한 균체의 감마선 조사에 의한 방사선 감수성을 조사하였다. 동결균체의 $D_{10}과\;12D_{10}$ 값으로 나타낸 방사선 감수성 Bacillus subtilis가 0.29kGy 와 3.48kGy, Bacillus cereus가 0.39kGy와 4.68kGy, Clostridium perfrigens가 0.46kGy와 5.52 kGy를 나타내었으며, 3kGy 와 5kGy에서의 불황성화계수는 6.52~10.34 및 10.87과 17.24였다. 이에 반해 우육에 접종 시킨 균체는 $D_{10}$값이 0.59~0.76kGy, $12D_{10}$값은 7.08~9.12kGy, 불활성화 계수는 3.95~8.47 범위로 동결균체에 비해 방사선 감수성 낮음을 알 수 있다.

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