한국식물병리학회지 (Korean Journal Plant Pathology)
- 제14권4호
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- Pages.339-344
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- 1998
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- 0256-8608(pISSN)
수박 덩굴마름병의 품종 저항성 검정과 감염 후 품종간 단백질 발현의 비교
Screening of Resistant Watermelon Cultivars Against Gummy Stem Blight Fungus, Didymella bryoniae, and Comparison of Protein Expression Between Cultivars After Infection
초록
Since the leaf inoculation procedures are time-consuming and require considerable growth chamber space, a rapid dioassay method for screening of pathogenicity of Didymella bryoniae, a casual agent of gummy stem blight in watermelon, was established in this paper. The method produced reliable results within 8 days ( 5 days for growing seedlings and 3 days for rapid disease response in the seedlings). After contaminants in the root of 4~5 day-old seedlings had been washed using sterilized water, 5 seedlings were dipped into a vial containing 12 ml of conidial suspension (106 cells/ml). After the vials were placed in a growth chamber (22