Korean Journal Plant Pathology (한국식물병리학회지)

The Korean Society of Plant Pathology (한국식물병리학회)
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RAPD Analysis of Host-specific Toxin (HST) Producing Alternaria species

기주특이적 독소를 생성하는 Alternaria 병원균군의 RAPD 분석

  • 김병련 (충남대학교 농과대학 응용생물화학학부) ;
  • 강희완 (농업과학기술원 세포유전과) ;
  • 유승헌 (충남대학교 농과대학 응용생물화학학부) ;
  • 이등정부 (일본 조취대학농학부) ;
  • 갑원철개 (조취대학농학부)
  • Published : 1998.02.01
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Abstract

RAPD analysis was performed from four host-specific toxin (HST) producing Alternaria, i.e., A. kikuchiana, A. mali, a. longipes and A. Longipes and A. alternata f. sp. lycopersici, nonpathogenic A. alternata and A. brassicicola to assess their phylogenetic relationship. DNA polymorphism was detected among species (pathotypes) of HST producing Alternaria by PCR amplification and differentiation of the species was recognized by RAPD analysis. Primer OPA-02 was the most profitable among 7 notificated primers for differentiation of the HST producing Alternaria species. UPGMA analysis of the RAPD bands from alternaria spp. revealed that HST producing Alternaria and nonpathogenic a. alternata are closely related.

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