Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 13 Issue 2
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- Pages.107-115
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- 1998
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Study on In Vitro Bovine Embryo Production with Follicular Oocytes Obtained via Ultrasound-guided Ovum Pick-up (OPU) and Slaughterhouse-derived(SHD) Ovary Aspiration in Korean Native Cows
한우에서 초음파 유래와 도축장 유래 난포란을 이용한 체외 수정란 생산에 관한 연구
Abstract
The present study was carried out for the comparative study on the collection of bovine follicular oocytes by ultrasound-guided ovum pick-up(OPU) and slaughterhouse-derived (SHD) ovary aspiration and in vitro production of bovine embryos with the follicular oncytes in Korean native cows. Bovine follicular nocytes were observed with a 6.5 MHz convex-array ultrasound transducer designed for intravaginal use and the oocytes were collected with the aspiration equipment attached to the ultrasonograph. Bovine ovaries were collected and transported in phosphate buffered saline from the local slaughterhouse, the follicular oocytes were collected by the aspiration method. The collected follicular oocytes in good quality were matured, fertilized and cultured in the media. The total number of the visible follicles and the recovery rate of follicular oocytes were increased in ultrasonography following follicle stimulating hormone(FSH) treatment in Korean native cows. The mean recovery rate of oocytes was 66.2, 52.8 and 41.7% in the FSH-OPU, non-treatment-OPU and SHD ovaries, respectively. The mean number of recorved oocytes per cow were not significantly(P<0.05) different between the FSH-OPU(14.0