KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Optimization of Fermentation Parameters for Heterologous Protein Productivity Enhancement with Pichia pastoris

Methylotrophic Yeast를 이용한 외래단백질 발현에서의 발효 변수 최적화

  • 강환구 (한남대학교 공과대학 화학공학과) ;
  • 이문원 (한남대학교 공과대학 화학공학과) ;
  • 전희진 (한남대학교 공과대학 화학공학과)
  • Published : 1998.06.01
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Abstract

The methylotrophic yeast, Pichia pastoris, is known to be a potential host to offer many advantages for production of recombinant proteins. Fermentation parameters were optimized to enhance the heterologous ${\beta}$-galactosidase productivity with P. pastoris. Optimum concentration of methanol, used as inducer, was observed to be 8 g/L and the extent of repression of AOX1 promoter by glycerol was lower than by glucose. The degradation of the gene product ${\beta}$-galactosidase by protease was inhibited as the pH increased from 5 to 8 and the yeast extract(1%) as nitrogen source increased expression level 4 times higher compared to yeast nitrogen base(1%) as nitrogen source increased expression level 4 times higher compared to yeast nitrogen base(1%). Induction method, in which methanol is just added to fermentation medium without centrifugation, was found to be as much effective as the one with centrifugation.

Keywords

References

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