KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Cell separation from high density culture broths of Alcaligenes eutrophus by using Al-based coagulants

Alcaligens eutrohus 고농도 배양액으로부터 알루미늄(Al)계 응집제를 이용한 세포분리

  • 조경숙 (이화여자대학교 환경공학과) ;
  • 류희욱 (숭실대학교 환경·화학공학과) ;
  • 정현우 (숭실대학교 환경·화학공학과) ;
  • 곽종운 (경기화학공업(주) 수처리연구실) ;
  • 장용근 (한국과학기술원 화학공학과 및 생물공정연구센터)
  • Published : 1998.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Cell recovery from high cell density broths of Alcaligenes eutrophus by pretreatment with aluminum-based coagulants such as aluminum sulfate, polyaluminum hydrooxide chloride silicate (PACS), and polyaluminum hydrooxide chloride (Hi-PAX) was carried out. Cells coagulated with coagulants could be successfully recovered above 95-99% by centrifugation or filtration. The optimum initial pH of fermentation broths for cell recovery was in the range of 10 to 12. Optimum coagulants dosage for cell recovery increased with increasing of cell concentrations (21-160 g/L). The optimum coagulant dosages to recover cells with more than 95% cell recovery by centrifugation for the cell concentrations ranged 21-160 g/L were as follows: aluminum sulfate, 416-1708 mg Al/L; PACS, 211-826 mg Al/L; Hi-PAX, 320-960 mg Al/L. At optimum conditions for the coagulation of cells, centrifugal forces for 95% of cell recovery were dependent on the cell concentration. The centrifugal forces at 82 g/L and 160 g/L of cell concentration were only 45${\times}$g and 1600${\times}$g, respectively.

Keywords

References

  1. Microbial polyesters Doi,Y.
  2. Biotechnol. Bioeng. v.43 Production of Poly(3-Hydroxybutyric Acid) by Fed-batch Culture of Alcaligenes eutrophus with Glucose Concentration Control Kim,B.S.;S.C.Lee;S.Y.Lee;H.N.Chang;Y.K.Chang;S.I.Woo
  3. Enzyme Microbial Technol. v.16 Production of Poly(3-Hydroxybutyric-co-3-Hydroxyvaleric Acid) by Fed-batch Culture of Alcaligenes eutrophus with Substrate Control Using On-line Glucose Analyzer Kim,B.S.;S.C.Lee;S.Y.Lee;H.N.Chang;Y.K.Chang;S.I.Woo
  4. Appl. Environ. Microbiol. v.61 Recovery and Characterization of Poly(3-Hydroxybutyric Acid) Synthesized in Alcaligenes eutrophus and Recobinant Escherichia coli Hahn,S.K.;Y.K.Chang;S.Y.Lee
  5. Biotechnol. Bioeng. v.55 Production of Poly(3-Hydroxybutyrate) by High Cell Density Fed-Batch Culture of Alcaligenes eutrophus with Phosphate Limitation Ryu,H.W.;E.K.Hahn;Y.K.Chang;H.N.Chang
  6. Recovery Processes for Biological Materials Filtration Pinheiro,H.;J.M.S.Cabral;J.F.Kennedy(ed.);J.M.S.Cabral(ed.)
  7. Recovery Processes for Biological Materials Sedimentation Pinheiro,H.;J.M.S.Cabral;J.F.Kennedy(ed.);J.M.Cabral(ed.)
  8. Recovery Processes for Biological Materials Centrifugation Pinheiro,H.;J.M.S.Cabral;J.F.Kennedy(ed.);J.M.S.Cabral(ed.)
  9. Recovery Processes for Biological Materials Membrane Separation Processes Mateus,M.;J.A.L.Santos;J.M.S.Cabral;J.F.Kennedy(ed.);J.M.S.Cabral(ed.)
  10. J. Water Poll. Control Fed. v.42 Algal Flocculation with Aluminum Sulfate and Polyelectrolytes McGarry,M.G.
  11. Water Chemistry Snoeyink,V.L.;D.Jenkins
  12. Aquatic Chemistry(3rd ed.) Stumm,W.;J.J.Morgan
  13. J. AWWA v.175 Ferric Chloride and Alum as Single and Dual Coagulants Johnson,P.N.;A.Amirtharajah
  14. Comprehensive Biotechnology The Principles, Applications, and Regulations of Biotechnology in Industrial, Agriculture and Medicine v.4 Precipitation and Coagulation in Waste Treatment Gregory,J.;M. Moo Young(ed.)
  15. Chemical Water and Wastewater treatment Ⅲ Influence of the Basicity of Polyaluminium Chlorides when Cleaning Municipal Wastewater Gillberg,L.;R.Klute(ed.);H.H.Hahn(ed.)
  16. Chemical Water and Wastewater treatⅢ Preliminary Evaluation of Polyferric Sulphate As a Coagulant for Surface Water Treatment Jiang,J.Q.;N.J.D.Graham;C.Harward;R.Klute(ed.);H.H.Hahn(ed.)
  17. Chemical Water and Wastewater treatment Ⅲ Coagulant Dosing Control Using a Model for Wastewater Coagulant Ratnaweera,H.;H.Blom;G.Aasgaard;R.Klute(ed.);H.H.Hahn(ed.)
  18. Biotechnol. Bioeng. v.25 New Concepts for Rapid Yeast Settling. I. Flocculation with an Inert Powder Weeks,H.G.;P.A.Munro;P.L.Spedding
  19. Process Chemistry for Water and Wastewater Treatment Benefield,L.D.;J.F.Judkins;B.L.Weand
  20. J. Coll. Sci. v.20 Some Studies of the Coagulation of Dispersed Clays with Hydrolyzing Salts Packham,R.F.
  21. J. Am. Water Works Assoc. v.60 Stoichiometry of Coagulation Stumm,W.;C.R.O'Melia