KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Optimum Conditions of Cellulose-Hydrolysis Reaction with Mixed Enzymes of Cellulase and $\beta$-Glucosidase

셀룰라아제와 베타글루코시다아제의 혼합효소를 사용한 섬유소-가수분해반응의 최적조건

  • 손민일 (명지대학교 공과대학 화학공학과) ;
  • 김태옥 (명지대학교 공과대학 화학공학과)
  • Published : 1998.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Optimum conditions of the cellulose-hydrolysis reaction with mixed enzymes(cellulase extracted from Penicellium funiculosum mixed with $\beta$-glucosidase extracted from Almod) were investigated to increase the production of glucose from cellulose. Experimental result showed that optimum conditions fro pH, activity ratio of $\beta$-glucosidase to cellulase, concentration of mixed enzymes, concentration of cellulose as a substrate, and temperature range were 4.2, 0.4, 0.8, U/mL, 40 g/L, and 37$\pm$3$^\circ C$, respectively. In these conditions, quantities of glucose productions by using mixed enzymes were larger than those by using cellulase at optimum conditions.

Keywords

References

  1. Biores. Technol. v.50 no.1 Ethanol from Lignocelluosic Biomass : Technology, Ecnomics, and Opportunities Wyman,C.E.
  2. TAPPI v.75 An Improved Process for Converting Cellulose to Ethanol Goldstein,I.S.;J.M.Ester
  3. Biotechnol. & Bioeng. v.25 Kinetic Study on Enzyme Hydrolysis of Cellulose by Cellulase from Trichoderma virde Ohmine,K.;H.Ooshima;Y.Harano
  4. World Congress Ⅲ of Chemical Engineering v.Ⅰ A Study on Enzymatic Hydrolysis of Cellulose in an Attrition Bioreactor Oh,K.D.;C.Kim
  5. 펄프·종이 技術 v.17 no.1 지·Pulp공장의 廢纖維素의 資源化에 關한 硏究(Ⅱ)-酸에 위한 sludge의 加水分解에 관하여 온두현;전병관;심규섭
  6. Biores. Technol. v.50 no.1 Ethanol Production from Lignocellulosics: Large Scale Experimentation and Economics Ballerini,D.;J.P.Desmarquest;J.Pourquie;F.Nativel;M.Rebeller
  7. Phytopathology v.63 Quantitative Changes in Structural Components of Conifer Woods During Decay by White-and Brown-Rot Fungi Kirk,T.K.;T.L.Highley
  8. Bioprocess Engineering: Basic Concepts Shuler,M.L.;F.Kargi
  9. Biotechnol. & Bioeng. v.20 Kinetics of Enzymatic Hydrolysis of Cellulose : Analytical Description of a Mechanistic Model Okazaki,M.;M.Moo-Young
  10. Biotechnol. & Bioeng. v.17 Enzymatic Breakdown of Water Insoluble Substrates Suga,K.;G.van Dedem;M.Moo-Young
  11. Biotechnol. & Bioeng. v.17 A Model for Diauxic Growth Van Dedem G.;M.Moo-Young
  12. Spectrophotometry and Spectrofluorimetry Harris,D.A.;C.L.Bashford
  13. Biotechnol. & Bioeng. v.27 Kinetic Modeling of the Enzymatic Hydrolysis of Preterated Cellulose Caminal,G.;J.Lopez-Santin;C.Sola
  14. Anal. Chem. v.31 Use of Dintrosalicylic Acid Reagent for Determination of Reducing Sugar Miller,G.L.
  15. 박사학위 논문, 화학공학과, 명지대학교 혼합효소에 의한 섬유소-가수분해반응 속도록과 공기리프트 생물반응기에서 글루코오스의 생산 손민일
  16. 化學藥品大辭典 文星明
  17. Enzyme Micro. Technol. v.4 Fungal and Other β-p-Glucosidases-Their Properties and Applications Woodward,J.;A.Wiseman
  18. Biochem. J. v.83 Almond-Emulsin β-p-Galactosidase Heyworth,R.;P.G.Walker
  19. Biochemical Engineering,2nd ed. Aiba,A.;A.E.Humphrey;N.F.Millis
  20. J. Ferment. Technol. v.57 no.1 A Model on Hydrolysis of Bagasse Cellulose by Enzyme from Trichoderma reesei QM 9414 Dwivedi,G.P.;T.K.Ghose
  21. Biotechnol. & Bioeng. v.17 Kinetic Studies on Insoluble Cellulose Cellulase System Huang,A.A.