Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Efficient Transformation of Klebsiella oxytoca by Electroporation

  • Ki Jun Jeong (Department of Chemical Engineering and BioProcess Engineerign Research Center, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Hyu (Department of Chemical Engineering and BioProcess Engineerign Research Center, Korea Advanced Institute of Science and Technology (KAIST) Department of Chemical Engineering and BioProcess Engineerign Research Center, Korea Advanced Institute of Science and Technology (KAIST) Department of Chemical Engineering and BioProcess Engineerign Research Center, Korea Advanced Institute of Science and Technology (KAIST))
  • Published : 1998.06.01
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Abstract

A protocol for the transformation of Klebsiella oxytoca by electroporation was developed. Preparation of competent cells at early exponential phase was most critical to obtain high transformation efficiency. The highest efficiency of 1.6$\times$106 transformants per $\mu\textrm{g}$ DNA(pBR322) could be obtained by electroporation of K. oxytoca cells prepared at the OD600 of 0.2 with 1.25$\mu\textrm{g}$ DNA at the filed strength of 2.5kV, the parallel resistance of 200$\Omega$ and capacitance of 25$\mu$F.

Keywords

References

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