Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Separation and Concentration of L-Phenylalanine using a Supported Liquid Membrane

  • Jeong Woo Choi (Department of Chemical Engineering, Sogang University) ;
  • Kyu (Department of Chemical Engineering, Sogang University Department of Chemical Engineering, Sogang University Material Development Div., Korea Institute of Geology, Mining and Material Department of Chemical Engineering, Sogang University)
  • Published : 1998.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The separation and concentration of L-phenylalanine (L-Phe) using a supported liquid membrane (SLM) is investigated. A cation complex agent, di-2-ethylhexyl phosphoric acid (D2EHPA), is used as a carrier in the SLM with n-Heptane as a solvent. The reaction order and equilibrium constant in the formation reaction of L-phe-carrier complex are obtained from the extraction experiment. A mathematical model for a carrier mediated counter transport process is proposed to estimate the diffusion coefficient of L-phe-carrier complexly in the liquid membrant. Permeation experiments of L-phe using a SLM are performed under various operating conditions and optimum conditions for the transport of L-phe are obtained. Concentration of L-phe in the strip phase against its concentration is observed. Transport rate of glucose through liquid membrane is less than that of L-phe in the competitive transport of L-phe and glucose. And the existence of glucose reduced the transport rate of L-phe. The performance of separation with continuous strip phase is increased due to the dilution effect in the strip phase.

Keywords

References

  1. Bioproducts and Bioprocess Production of L-phenylalanine using enzymes Tosa, T.;M. Fiechter(ed.)
  2. J. Membr. Sci v.100 Analysis of permeation rate of cobalt ions across a supported liquid membrane containing HEH(EHP) Youn, I. J.;Y. Lee;W. H. Lee
  3. J. Membr. Sci v.125 Analysis of Co-Ni separation by a supported liquid membrane containing HEH(EHP) Youn, I. J.;Y. Lee;J. Jeong;W. H. Lee
  4. AIChE J v.17 Permeation through liquid surfactant membrane Li, N. N.
  5. Biotechnol. Bioeng v.35 Separation of L-valine from fermentation broths using supported liquid membrane Debly, P.;M. Minier;H. Renon
  6. Biotechnol. Bioeng v.35 A liquid emulsion membrane process for the separation of amino acid Itoh, H.;M. P. Thien;T. A. Hatton;D. I. C. Wang
  7. AIChE J v.34 A liquid emulsion membrane process for the separation of amino acid Majumdar, S.;A. K. Guha;K. K. Sirkar
  8. J. Membr. Sci v.64 Selective transport of calcium ion from a mixed cation solution through an HDEHP/n-dodecane supported liquid membrane Nair, S. N.;S. T. Hwang
  9. Engineering Optimization Methods and Applications Reklaitis, G. V.
  10. Diffusional Mass Transfer Skelland, A. H. P.
  11. J. Membr. Sci v.31 Transport of zinc through a supported liquid membrane using di(2-ethylhexyl) phosphoric acid as a mobile carrier Huang, T. C.;R. S. Juang
  12. Sep. Sci. Technol v.17 no.9 Transport of $Eu^{3+}$ through a bis (2-ethylhexyl)-phosphoric acid, n-dodecane supported liquid membrane Danesi, P. R.;E. P. Horwitz;P. Rickert
  13. J. Membr. Sci v.7 Coupled transport membranesⅢ: The rate limiting step in uranium transport with a tertiary amine Bobcock, W. C.;R. W. Baker;E. D. Lachapelle;K. L. Smith
  14. Chem. Rev v.1 The theory of membrane equilibria Donnan, F. G.
  15. Principles of Biochemistry Lehninger, A. L.
  16. J. Membr. Sci v.64 Carrier-mediated transport of uranyl ions across tributyl phos-phate-dodecane liquid membranes Shukla, J. P.;S. K. Misra