Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Enzymatic Determination of Glucose Using Soybean Sprouts Peroxidase.

콩나물 Peroxidase를 이용한 포도당의 효소적 분석

  • 이민경 (동아대학교 식품영양학과)
  • Published : 1998.08.01
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Abstract

Soybean sprouts peroxidase can be used for enzymatic determination of glucose. Peroxidase from soybean sprouts was purified by ammonium sulfate precipitation and DEAE Sephacel column chromatography. The glucose could be quantitatively assayed by using glucose oxidase and soybean sprouts peroxidase. The optimum pH and temperature for glucose assay were of pH 5.5 and $40^{\circ}C$, respectively. The relationship between absorbance and glucose concentration was linear. And also the relationship between absorbance and reaction time was linear. The reducing agents such as L-cysteine, dithiothreitol inhibited the glucose assay by glucose oxidase and soybean sprouts peroxidase.

콩나물 줄기로부터 추출, 정제한 peroxidase는 glucose oxidase와 함께 guaiacol을 기질로 사용하여 포도당의 분석에 사용되었다. peroxidase는 DEAE-Sephacel ion exchange column chromatography를 통해 얻은 분획이 조효소액에 비해 specific activity가 10.8배 증가되었고 수율은 11.3%였다. 정제된 peroxidase와 glucose oxidase를 이용한 포도당 분석의 최적 pH는 5.5였고 최적온도는 $40^{\circ}C$로 나타났으며 포도당 양의 증가에 따라 효소활성은 증가되었으며 반응시간과의 관계에서도 직선을 보여 주었다. 그리고 L-cysteine과 dithiothreitol과 같은 환원제는 포도당 분석에 이용되는 glucose oxidase와 콩나물 peroxidase의 활성을 저해하는 것으로 나타났다.

Keywords

References

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