근육의 전기자극에 의한 X선 회절 분석연구

A Study on the X-ray Diffraction of the Muscle by the Electrical Stimulation

  • 발행 : 1998.08.01

초록

A considerable change observed in X-ray diffraction during the muscle contraction was that the movement of myosin head and conformational change of contractile monecules were occurred in the muscle contraction. Time slice requires tension peak after the onset of stimulation and the height of tension peak depends on the number of twitch cycle. The intensity of I$_{11}$, I$_{10}$, 143${\AA}$ reflection is measured with 5ms time resolution and is recorded in isometric tension. The peak height of I$_{11}$ and 143${\AA}$ intensity is changed after the onset of a stimulation I$_{i}$, and the length of twitch is shortened by successive twitches in the case of stimulation TI$_{i}$. On the other hand, the peak height of I$_{11}$ and 215${\AA}$ intensity starts to decrease at the 1st twitch and remains constant at low peak hight without appreciable recovery during the contraction term. In the case of uccessive twitch stimulation, the myosin heads of muscle are once moved from their resting position and never returned to their initial position.

키워드

참고문헌

  1. Cold Spring Harbor Symp. Quant. Biol. v.37 Mechanical transients and the origin of muscular force Huxley, A. F.;Simmons, R. M.
  2. J. Mol. Biol. v.30 The low-angle x-ray diagram of vertebrate striated muscle and its behaviour during contraction and rigor Huxley, H. E.;Brown, M.
  3. Adv. Exp. Med. Biol. v.266 X-ray diffraction studies on muscle during shortening and their implications concerning crossbridge behaviour Huxley, H. E.;Simmons, R. M.;Farqi, A. R.;Kress, M.
  4. J. Mol. Biol. v.158 Time - resolved x-ray diffraction studies of the myosin layer-line reflections during muscle contraction Huxley, H. E.;Farqi, A. R.;Kress, M.;Bordas, J.;Koch, M. H. J.
  5. Nucl. Instrum. Methods v.208 Design of a small-angle x-ray diffractometer using synchrotron radiation at the Photon Factory Amemiya, Y.;Wakabayashi, K.;Hamanaka, T.;Wakabayashi, T.;Matsushta, T.;Hashizume, H.
  6. Handbook on Synchrotorn Radiation v.4 Progress in x-ray synchrotron diffraction studies of muscle contraction Wakabayashi, K.;Amemiya, Y.
  7. Adv. Biophys. v.27 Dynamic x-ray diffraction of skeletal muscle contraction structural change of actin filaments Wakabayashi, K.;Tanaka, H.;Saito, N.;Motiwaki, N.;Ueno, Y.;Amemiya, Y.
  8. J. Mol. Biol. v.208 Structural change in the thin filament during activation studied by x-ray diffraction by highly stretched skeletal muscle Yagi, N.;Matsubara, I.
  9. J. Muscle Res. Cell Motil. v.11 Tension and stiffness of frog muscle fibers at full filament overlap Bagni, M. A.;Cecchi, G.;Colomo, F.;Poggesi, C.
  10. Biophys. J. v.68 Effect of Stretch and Release on Equatorial X-ray Diffraction During a Twitch Contraction of Frog Skeletal Muscle Iwamoto, H.;Kobayashi, T.;Amemiya, Y.;Wakabayashi, K.
  11. J. Mol. Biol. v.188 Structural changes during activation of frog muscle studies by time-resolved x-ray diffraction Kress, M.;Huxley, H. E.;Faruqi, A. R.;Hendrix, J.
  12. Biophys. J. v.64 Viscoelasticity of the sarcomere matrix of skeletal muscle contraction the actin-myosin composite filament is a dual-stage molecular spring Wang, K.;McCarter, R.;Wright, J.;Beverly, J.;Ramirez-Mitchel, R.
  13. Photon Factory Act. Rep. v.10 Detection of the spacing changes of muscle thin filaments during force generation by x-ray diffraction Wakabayashi, K.;Saito, H.;Kobayashi, T.;Ueno, Y.;Tanaka, H.
  14. J. Cell Biol. v.127 Structural changes in muscle crossbridge accompanying force generation Hirose, K.;Murray, T. J. M.;Franzini-Armstrong, C.;Goldman, Y. E.
  15. J. Mol. Biol. v.231 An x-ray diffraction study of frog skeletal muscle during shortening near the maximum velocity Yagi, H.;Takemori, S.;Yamaguchi, M.