Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidative Effect of Silybin Purified from Silybum marianum on modification of Human Low Density Lipoprotein

엉겅퀴에서 분리정제한 silybin의 Human Low Density Lipoprotein 수식에 대한 항산화 효과

  • Published : 1998.08.01
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Abstract

This study was initiated to antioxidant activity of silybin on oxidation of human low density lipoproteins(LDL). Siltbin was extracted from Silybum marianum by the combination of fractionation and it was futher purified by silica gel column chromatography, and isolated active substances were identified silybin by IR, NMR and GC-MS. siltbin inhibited the ozidation of human low density lipoprotein(LDL) mediated by 5$^{\mu}$m CU $^{2+}$ ion in a dose dependent manner. LDL oxidation by congugated dines formation was completely inhibited by silybin at a concentration of 5$^{\mu}$M. The results provide a possibility that silybin might protect LDL against oxidation in atherosclerotic lessions.

Keywords

References

  1. Biochem. Med. Metabol. Biol. v.39 Effects of flavonoids on nonenzymic lipid peroxidation. Structure activity relationship Ratty, A. K.;Das, N. P.
  2. Plant Flavonoids in Biology and Medicine, Biochemical, Pharmacological and Structural Activity Relationships The metabolism of flavonoid compounds in mammals Hackett, A. M.
  3. Plant Flavonoids in Biology and Medicine II, Biochemical, Cellular, and Medicinal Properties Crystal and molecular structure of flavonoids Cody, V.
  4. J. Food Technol. v.11 Flavonols and flavones in plants. A review Herrmann, K.
  5. World Rev. Nutr. Diet. v.24 The flavonoids. A class of semi-essential food components, Their role in human nutrition Kuhnau, J.
  6. Plant Flavonoids in Biology and Medicine, Biochemical, Pharmacological and Structure -Activity Relationships Flavonoids in the human diet Pierpoint, W. S.
  7. Brit. J. Nutr. v.71 Degradation of polyphenols(catechin and tannic acid) in the rat intestinal tract. Effect on colonic fermentation and faecal output Bravo, L.;Abia, R.;Eastwood, M. A.;Saura-Clixto, F.
  8. Biochem. Pharmacol. v.20 Studies on flavonoid metabolism. Absorption and metabolism of(+)-catechin in Man Das, N. P.
  9. Eur. J. Clin. Pharmacol. v.9 Disposition of quercetin in Man after single oral and intravenous doses Gugler, R.;Leschik, M.;Dengler, H. J.
  10. Plant flavonoids in biology and medicine-biochemical, pharmacological, and structure Cody, V.(ed.);Middleton, E.(ed.);Harborme, J. B.(ed.)
  11. J. Natl. Prod. v.53 Inhibitory effects of tannins on HIV reverse transcriptase and HIV replication in H9 lymphocyte cells Nonaka, G.;Nishioka, L.;Nishizawa, M.;Yamagishi, T.;Kashiwada, Y.;Dutschman, G. E.;Bodner, A. J.;Kilkuskie, R. E.;Cheng, Y. C.;K.H. Lee
  12. Lancet v.342 Dietary antioxidant flavonoids and risk of coronary heart disease. The Zutphen elderly study Hertog, M. G. L.;Hollman, P. C. H.;Katan, M. B.;Kromhout, D.
  13. Food Technol. Possible mechanisms for the protective role of antioxidants in wine and plant foods Kinsella, J. E.;Frankel, E.;German, B.;Kanner, J.
  14. Crit. Rev. Food Sci. Nutr. v.29 Antioxidants/antimutagens in food Namiki, M.
  15. Nutr. Cancer v.20 Intake of potentially anticarcinogenic flavonoids and their determinats in adults in The Netherlands Hertog, M. G. L.;Hollman, P. C. H.;Katan, M. B.;Kromhout, D.
  16. Biochem. Pharmacol. v.39 Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages De Whalley, C. V.;Rankin, S. M. H.;oult, J. R. S.;Jessup, W.;Leake, D. S.
  17. Arteriosclerosis. v.3 Enhanced macrophage degradation of biologically modified low density lipoprotein Henriksen, T.;Mahoney, E. M.;Steinberg, D.
  18. Proc. Natl. Acad. Sci., USA v.78 Enhanced macrophage degradation of low density lipoprotein previously incubated with cultured endothelial cells. Recognition by the receptor for acetylated low density lipoprotein Henriksen, T.;Mahoney, E.;Steinberg, D.
  19. Arterloscerosis v.4 Endothelial and smooth muscle cells alter low density lipoprotein in vitro by free radical oxidation Morel, D. W.;Docorleto, P. E.;Chisolm, G. M.
  20. Proc. Natl. Acad. Sci., USA v.84 Oxidatively modified low density lipoproteins. A potential role in recruitment and retention of monocyte/macrophages during athero-genesis Quinn, M. T.;Parthasarathy, S.;Fong, L. G.;Steinberg, D.
  21. Klin Wochenschr. v.69 Oxygen radicals and atherosclerosis Carpenter, K. L. H.;Brabbs, C. E.;Mitchinson, M. J.
  22. Curr. Opin. Lipidol. v.1 Free radicals, lipid peroxidation and atherosclerosis Bruckdorfer, K. R.
  23. Am. J. Clin. Nutr. v.53 Role of vitamin E in preventing the oxidation of low-density lipoprotein Esterbauer, H.;Puhl, H.;Dieber-Rotheneder, M.;Striegl - Waeg, G. G.
  24. Ann. N. Y. Acad. Sci. v.570 The role of vitamin E and carotenoids in preventing oxidation of low density lipoproteins Esterbauer, H.;Streigel, G.;Puhl, H.;Oberreither, S.;Rotherneder, M.;El-Saadani, M.;Urgens, G.
  25. Scand. J. Gastroenterol. v.17 Effect of silybin on chemical, functional, and morphological alterations of the liver Salmi, H. A.;Sarna, S.
  26. Arzneimitteforsch v.23 Die Antihepatotoxische Wirkung von parenteral verabreichtem silybin bei der Leberchadigung der Ratte durch CCI₄ Rauen, H. M.;Schriewer, H.
  27. Arzneimittelf-orsch. v.21 Die antihepatotoxische Wirkung von silybin bei experimentellen Leberschagungen der Ratte durch Tatrachlorkohlen stoff, D-Galaktosamin und Allylalkohol Rauen, H. M.;Schriewer, H.
  28. Fund. Clin. Pharmacol. v.3 Prevention of CCl₄-induced cirrhosis by silymarin Mourelle, M.;Murrel, P.;Favari, L.;Franco, T.
  29. 경성대학교 대학원 박사학위논문 엉겅퀴에서 정제한 Silymarin의 항산화 효과 이백천
  30. J. clin. Invest. v.34 The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum Havel, R. J.;Eder, H. A.;Bragdon, J. H.
  31. Free Rad. Res. Commun. v.6 Continuos monitoring of in vitro oxidation of human low density lipoprotein Esterbauer, H.;Striegl, G.;Puhl, H.;Rotheneder, M.
  32. Biochem. Med. v.15 A simple flavorometric assay for lipoperoxide in blood plasma Yaki, K.
  33. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;Rosebrough, N. J.;Farr, A. L.;Randell, R. J.
  34. The Systematic Identification of Flavonoids Mabry, T. J.;Markham, K. R.;Thomas, M. B.
  35. The chemistry of flavonoid compound Geisman, T. A.
  36. Yakugagu zasshi v.95 Studies on constituent of Silybum marianum(L.) Takemoto, T.;Ikegawa, S.;Nomoto, K.
  37. Current Opin. Lipidologe v.3 Antioxidants and atherosclerosis Jialal, I.;Scaccini, C.
  38. J. Clin. Invest. v.77 Probucol inhibits oxidative modification of low density lipoprotein Parthasarathy, S.;Young, S. G.;Witztum, J. L.;Pittman, R. C.;Steinberg, D.
  39. Ann. Med. v.23 Effect of antioxidants on oxidative modification of LDL Esterbauer, H.;Puhl, H.;Dieber-Rotheneder, M.;Waeg, G.;Rable, H.
  40. Planta Med. v.50 Antihepatotoxic actions of flavonolignans from silybum marianum fruits Nikino, N.;Kiso, Y.;Wagner, H.;Fiebig, M.
  41. Flavonoids in biology and medicine III -Curent issues in flavonoids research Das, N. P.(ed.)