Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Identification of Mariner-Like Element(MLE) Gene from Nombyx mori.

누에에서의 Mariner 유사 전이인자유전자의 동정

  • Lee, Jin-Sung ;
  • Hwang, Jae-Sam ;
  • Kim, Yong-Sung ;
  • Suh, Dong-Sang ;
  • Kwon, O-Yu
  • 이진성 (생명공학연구소 게놈사업단) ;
  • 황재삼 (농촌진흥청 잠사곤충연구소) ;
  • 김용성 (생명공학연구소 게놈사업단) ;
  • 서동상 (성균관대학교 유전공학과) ;
  • 권오유 (충남대학교 해부학교실)
  • Published : 1998.06.01
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Abstract

We have cloned an internal fragment of the putative transoisase gene of MLE in the silkworm, Bombyx mori, using PCR method with degenerative oligonucleotide primers designed to represent regions of amino acids encoding transposase. The resulting PCR clone, designed as BmoMAR, cords a partial ORF(152 a.a.) of MLE in which interrupted by five stop codons, and the sequence of its deduced amino acids showed 37% homology with Mos1, an active mariner, from Drosophila mauritiana. Furthermore, the BmoMAR exhibits nucleotide and amino acid homology with 59% and 37% from Apis mellifera and D. mauritiana 7.9 clone, respectively. This result strongly that a MLE is present in the genome of B. mori.

이미 밝혀져 있는 mariner 전이인자의 전이효소를 암호화하는 부위에 대하여 퇴화성 primer를 사용하여 PCR 방법에 의해 누에(Bombyx mori)에서 ariner 유사 전이닌자의 잠정적인 전이효소 부위를 클로닝 하였다. BmoMAR로 망명된 이 PCR 클론으로부터 추론된 아미노산은 152개로 다섯 개의 종결코돈이 삽입되어 있었으며, Drosophila mauritiana의 active Mos 1에 37%의 아미노산 상동성을 보였다. 또한, 기존의 곤충들에서 밝혀진 mariner-like element에 대한 상동성은 DNA 수주에서는 Apis mellifera에 59% 그리고 아미노산 수준에서는 D. mauritiana 7.9 clone에 37% 상동성을 보였다. 이 결과는 mariner-like element가 B. mori에도 존재하고 있지만. 이들 전이인자의 전이효소를 암호화하는 부위에 종결코돈이 발견되는 것으로 보아서 비활성 전이인자 혹은 일존의 selenoprotein으로 추정된다.

Keywords

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