Effect of Pine Needle Extract (PNE) on Physilolgical Activity of SD Rats III. Feeding Effect of PNE on Fluidity and Neurotransmitter-Related Enztmes in Brain Membranes of SD Rats

흰쥐의 생리활성에 미치는 송엽(松葉) 추출물(PNE)의 영향 III. 뇌세포막의 유동성 및 신경전달관련 효소의 활성에 미치는 PNE의 투여효과

  • 최진호 (부경대학교 식품생명과학과 생화학연구실) ;
  • 김정화 (부경대학교 식품생명과학과 생화학연구실) ;
  • 김동우 (부경대학교 식품생명과학과 생화학연구실) ;
  • 황찬호 (부경대학교 식품생명과학과 생화학연구실) ;
  • 김대익 (부경대학교 식품생명과학과 생화학연구실) ;
  • 이종수 (부경대학교 식품생명과학과 생화학연구실)
  • Published : 1998.04.01

Abstract

To investigate the effect of pine needle extract (PNE) on membrane fluidirt and neurotransmiter-related enzymes in brain of Spragu-Dawley(SD), male SD rats were fed basic diets (control group), and experimantal diets (PNE group)with 0.5% and 0.1% fo PNE for 6 weeks. pine (pinus tabulaeformis C$_{ARR}$ is one of the popular plant drugs which has used as a medicine in Asia. Cholesterol levels in brain mitochondria of 0.5%-PNE and 0.1%-PNDE groups were significantly decreased in 15% and 25%, respectively, compared with control group, but cholesterol levels in brain microsomes of these PNE groups howed almost no change compared with control group. Lipofuscin accumulations in brain membranes of 0.5%-PNE and 0.1%-PNE groups were sgnificantly inhibited in 18% and 21%, respectively, compared with control group. Brain memberance fluidity was also activated in 50% and 100% by the administration of 0.5%-PNE and 0.1%-PNE. higher acetylcholinesterase(15% and25%) and lower monoamine oxidase B (25% and 15%0 activities were effectively modulated by the administration of 0.5%-PNE and 0.1%-PNDE. These results suggest that more beneficial effects such as inhibition of cholesterol and lipofuscin, increase of membrane fluidity, higher acetylcholinesterase and lower monoamone oxidase activities in brain membranes of SD rats may be effectively modulated by administration of pine needle extract (PNE).

Keywords

References

  1. 韓國食經大典 李盛雨
  2. 原色和漢藥圖鑑(下) 難波恒雄
  3. 한국노화학회지 v.1 no.1 솔잎 첨가식이가 흰쥐의 혈청 지방질 대사에 미치는 영향 김종대;윤태헌;최면;임경장;주진순;이상영
  4. 한국영양식량학회지 v.25 no.3 솔잎 추출물이 고지방식이를 급여한 흰쥐의 혈청과 간장의 지질 조성에 미치는 영향 강윤한;박용곤;하태열;문광덕
  5. 한국영양식량학회지 v.25 no.3 솔잎 추출물이 지방식이를 급여한 흰쥐의 혈청, 간장의 효소 및 간조직 구조에 미치는 영향 강윤한;박용곤;하태열;문광덕
  6. Murat Res Aug. v.347 no.3-4 Study on the antimutagenic effect of pine needle extract. Kong, Z.;Liu, Z.;Ding, B.
  7. 한국생명과학학회지 v.7 no.4 흰쥐(SD rats)의 생리활성이 미치는 송엽(松葉) 추출물(PNE)의 영향 Ⅰ. 혈청중의 지질 및 산소라디칼 대사에 미치는 PNE의 투여효과. 최진호;김동우;김정화;김경석;이종수
  8. 한국생명과학학회지 v.8 no.1 흰쥐(SD rats)의 생리활성이 미치는 송엽(松葉) 추출물(PNE)의 영향 Ⅱ. 뇌세포막의 산소라디칼 및 그 제거 효소의 활성에 미치는 PNE 의 투여효과. 최진호;김동우;김정화;김경석;이종수
  9. Age v.19 Analysis of lipid composition and hydroxyl radicals in brain membranes of senescence-accelerated mice Choi, J. H.;Yu, B. P.
  10. J. Biol. Chem. v.193 Protein measurement with the Fohlin-Phenol reagent. Lowry, O. H.;Roseborough, N. J.;Farr, L. A.;Randall, R. J.
  11. J. Lipid. Res. v.14 Determination of cholesterol using o-phthalaldehyde Rudel, L. L.;Morris, M. D.
  12. Free Rad. Biol. & Med. v.18 no.2 Brain synaptosomal afing : free radicals and membrane fluidity Choi, J. H.;Yu, B. P.
  13. Mech Age Dev v.65 Effect of agerelated lipid peroxidation on membrane fluidity and phospholipase A₂ : Modulation by dietary restriction. Yu, B. P.;Suescun, E. A.;Yang, S. Y.
  14. Anal Biochem v.52 Measurement of fluorescent lipid peroxidation products in biological systems and tissue Fletcher, B. L.;Dillard, C. J.;Tappel, S. A. L.
  15. Neuropharmacol v.26 no.6 Comparison of the effects of two inhibitors on brain cholinesterase. Hallak, M.;Giacobini, E. A.
  16. Proc. Natl. Acad. Sci. v.84 Correlation of 1-methyl-4-phenyl-1, 2, 3, 6-tertrahydropyridine neurotoxicity with blood-brain barrier monoamine ocidase activitu. Kalaria, R. N.;Mithchell, M. J.lHarik, S. I.
  17. Principles and Procedures of stastistics. Steel, R.G.D.;Torrie, J. H.
  18. Am. J. Clin. Nutr. v.22 Serum cholesterol and triglyceride. An epidemiologycal adn pathogenetic interpretation. Schilling , R. J.;Christakis, G.;Orbach, A.;Becker, W. H.
  19. Chemistry and physics of Lipids v.44 Linpofuscin and lipofuscin-like substances. Tsuchida, M.;Miura, T.;Alsara, K.
  20. Brain Res. Bull. v.22 The ingluence of cholesterol on synaptic fluidity and dopamine uptake. Maguire, P. A.;Druse, M. J.
  21. J. Pharmacol. Exp. Ther. v.229 Membrane properties and aging : In vivo and in vito effects of ethanol on synaptosomal β-aminobutyric acid(GABA) release. Strong, R.;Wood, W. G.
  22. Neurobiology of Aging Age changes in the human for some enzymes associated with metabolism of catecholamines, GABA, and acetylcholine MeGeer, E. G.;MCGeer, P. L.;Irdy JM(ed.);Brizzee(ed.)
  23. Neuroniol Aging v.1 Age-related alterations in the rodent brain cholinergic system and behabior. Strong, R.;Hsu, L.;Bartus, R. T.;Enna, S. J.
  24. Age v.4 Deficiencies in the cholinergic nervous system of the rat cerebrum ad a function of age. Sastry, B. V.;Jason, V. E.;Jaiswal, N.;Tayeb, O. S.