Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Regulation of Two Soluble Forms of Brain Glutamate Dehydrogenase Isoproteins by Protein Kinases

  • Lee, Jong-Weon (Department of Parasitology and Institute of Tropical Medicine, College of Medicine, Yonsei University) ;
  • Choi, Soo-Young (Department of Genetic Engineering, Division of Life Sciences, Hallym University) ;
  • Cho, Sung-Woo (Department of Biochemistry, College of Medicine, University of Ulsan)
  • Published : 1998.06.01
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Abstract

We isolated two soluble forms of glutamate dehydrogenase isoproteins, GDH I and GDH II, from bovine brain. The regulation of GDH I and GDH II by phosphorylation and dephosphorylation has been examined in various conditions. There were dose- and time- dependent activation of the GDH isoproteins when phosphorylated by cAMP-dependent protein kinase. The phosphorylated GDH had 1.1 mol of covalently bound phosphate/mol of subunit and a 2-fold increased specific activity. The phosphorylated amino acid was identified as serine. When treated with alkaline phosphatase, the activities of the phosphorylated GDH isoproteins were reduced in dose and time dependent manner and returned to those of unphosphorylated enzymes. There were no significant differences between GDH I and GDH II in their sensitivities to the action of phosphorylation and dephosphorylation demonstrating that the microenvironmental structures of the phosphorylation site in GDH isoproteins are similar to each other, These results results suggest that the inter-conversion between less active form of brain GDH isoproteins and more active form is regulated by phosphorylation through cAMP-dependent protein kineses.

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References

  1. Invest Ophthalmol & Visual Sci v.33 Partial deficiient glutamate dehydrogenase activity and attenuated oscillator potentials in patients with spinocerebellar degeneration Abe T;Ishiguro SI;Saito H;Kiyosawa M;Tarnai M
  2. J Neurol Neurosurg Psych v.51 Leukocyte glutamate dehydrogenase activity in patients with degererative neurological disorders Aubby D;Saggu HK;Jenner P;Quinn NP;Harding AE;Marsden CD
  3. J Mol Biol v.228 Structural consequences of sequence patterns in the fingerprint region of the nucleotide binding fold: implications for nucleotide specificity Baker PJ;Britton KL;Rice DW;Rob A;Stillman TJ
  4. Mol Cell Biol v.17 Mitochondiral glutamate dehydrogenase from Leishmania tarentolae is a guide RNA-binding protein Bringaud F;Stripecke R;Frech GC;Freedland S;Turck C;Byrne EM;Simpson L
  5. Proc Natl Acad Sci USA v.94 Late mernory-related genes in the hippocampus revealed by RNA fingerprinting Cavallaro S;Meiri N;Yi CL;Musco S;Ma W;Goldberg J;Alkon DL
  6. Biochemistry v.35 Identificatin of a peptide of the guanosine triphosphate binding site within brain glutamate dehydrogenase isoproteins using 8-azidoguanosine triphosphate Cho S-W;Ahn J-Y;Lee J;Choi SY
  7. Biochimie v.78 Modification of brain glutamate dehydrogenase isoproteins with pyridoxal 5'-phosphate Cho S-W;Lee JE
  8. Eur J Biochem v.233 Two doluble forms of glutamate dehydrogenase isoproteins from bovine brain Cho S-W;Lee J;Choi SY
  9. J Neurochem v.46 Purification and characterization of a soluble and a particulate glutamate dehydrogenase from a rat brain Colon A;Plaitakis A;Perakis A;Berl S;Clarke DD
  10. J Neurol Neurosurg Psych v.33 Low leukocyte glutamate dehydrogenase activity does not correlate with a particular type of multiple system atrophy Duvoisin RC;Nicklas WJ;Ritchie V;Sage J;Chokroverty S
  11. Gene v.192 Identification of a new member (ZNF183) of the ring finger gene family in Xq24-25 Frattini A;Faranda S;Bagnasco L;Patrosso C;Nulli P;Zucchi I;Vezzoni P
  12. J Biol Chem v.253 Phosphorylation of NAD-dependent glutamate dehydrogenase from yeast Hemmings BA
  13. J Biol Chem v.255 Purification and properties of the phospho and dephospho forms of yeast NAD-dependent glutamate dehydrogenase Hemmings BA
  14. J Biol Chem v.264 Characterization of glutamate dehydrogenase isoproteins purified from the cerebellum of normal subjects and patients with degenerative neurological disorders, and from human neoplastic cell lines Hussain MH;Zannis VI;Plaitakis A
  15. J Neurochem v.69 Essential active-site lysine of brain glutamate dehydrogenase isoproteins Kim SW;Lee J;Song MS;Choi SY;Cho SW
  16. J Neurol Sci v.74 Glutamate dehydrogenase and its isoenzyme activity in olivopontocerebellar atrophy Konagaya Y;Konagaya M;Takayanagi T
  17. Annu Rev Biochem v.48 Phosphorylation-dephosphorylation of enzymes Krebs EG;Beabo JA
  18. Proc Natl Acad Sci USA v.85 Isolation and characterization of cDNA clones encoding human liver glutamte dehydrogenase: evidence for a small gene family Mavrothalassitis G;Tzimagiorgis G;Mitsialis A;Zannis VI;Plaitakis A;Papamatheakis J;Moschonas NK
  19. Arch Biochem Biophys v.319 Comparison of the primary structure of nuclear and mitochondrial glutamate dehydrogenase from bovine liver McDaniel HG
  20. Nature v.263 Duplication of biochemical changes of Huntington's chorea by intrastriatal injection of glutamic and kainic acid McGeer EG;McGeer PL
  21. Genomics v.16 The human glutamate dehydrogenase gene family: gene organization and structural characterization Michaelidis TM;Tzimagiorgis G;Moschonas N;Papamatheakis J
  22. Eur J Biochem v.68 The purification and properties of rabbit skeletal muscle glycogen synthase Nimmo HG;Proud CG;Cohen P
  23. J Struct Biol v.120 Crystallization and characterization of bovine liver glutamate dehydrogenase Peterson PE;Pierce J;Smith TJ
  24. Science v.216 Abnormal glutamate dehydrogenase deficiency in three patients with spinocerebellar syndrome Plaitakis A;Berl S;Yahr MD
  25. Can J Neurol Sci Suppl v.3 Glutamate dehydrogenase deficiency in cerebellar degenerations: clinical, biochemical and molecular genetic aspects Plaitakis A;Flessas P;Natsiou AB;Shashidharan P
  26. J Biol Chem v.268 Identification of bovine glutamate dehydrogenase as an RNA-binding protein Preiss T;Hall AG;Lightowlers RN
  27. FEBS Lett v.367 The mRNA-binding protein COLBP is glutamate dehydrogenase Preiss T;Sang A;Chrzanowska-Lightowlers ZMA;Lightowlers RN
  28. Trends Biochem Sci v.22 Glutamate dehydrogenase: an organelle-specific mRNA-binding protein Preiss T;Chrzanowska-Lightowlers ZM;Lightowlers RN
  29. Biochem J v.295 A membrane-bound form of glutamate dehydrogenase possesses an ATP-dependent high-affinity microtubule-binding activity Rajas F;Rouset B
  30. J Biol Chem v.271 Involvement of a membrane-bound form of glutamate dehydrogenase in the association of lysosomes to microtubles Rajas F;Gire V;Rouset B
  31. J Biol Chem v.269 Novel human glutamate dehydrogenase expressed in neural and testicular tissues and encoded by an X-linked intronless gene Shashidharan P;Michaelidis TM;Robakis NK;Kresovali A;Papamatheakis J;Plaitakis A