Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Expression of Bcl-2 Protein in Ischemia-Reperfused Myocardium of Rabbit

가토 허혈-재관류 심근에서의 Bcl-2 단백의 발현

  • 류재욱 (단국대학교 의과대학 흉부외과학교실) ;
  • 김삼현 (단국대학교 의과대학 흉부외과학교실) ;
  • 서필원 (단국대학교 의과대학 흉부외과학교실) ;
  • 박성식 (단국대학교 의과대학 흉부외과학교실) ;
  • 최창휴 (단국대학교 의과대학 흉부외과학교실) ;
  • 류경민 (단국대학교 의과대학 흉부외과학교실) ;
  • 김영권 (단국대학교 의과대학 내과학교실) ;
  • 박이태 (청주성모병원 흉부외과) ;
  • 김성숙 (울산대학교 의과대학 병리학교실)
  • Published : 1998.10.01
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Abstract

Background: Myocardial cell death after myocardial infarction or reperfusion is classified into necrosis and apoptosis. Bcl-2 protein is a cytoplasmic protein, which inhibits apoptosis and is expressed in acute stage of myocardial infarction but not in normal heart. This study was performed to investigate whether Bcl-2 protein was expressed respectively to the reperfusion time. Materials and methods: Thirty nine New Zealand white rabbits weighing 1.5-4.8 kg (mean, 2.9kg) were alloted into 7 groups (n=5 in each group) which underwent left anterior descending coronary artery(LAD) occlusion for 30 minutes, followed by reperfusion. The animals were sacrificed at 1, 4, 8, 12, 24 hours, and 3, 7 days after occlusion. Ventricle was excised immediately after intervention. Tissues were fixed in 10% buffured formalin and embedded in paraffin. Bcl-2 protein was detected by immunohistochemical stain with using monoclonal antibody against Bcl-2 protein. Results: The positive immunohistochemical reactivity for Bcl-2 protein was observed in 12, 24 hours, and 3 days reperfusion groups. Bcl-2 protein was detected in salvaged myocytes surrounding the infarcted area. Conclusions: Bcl-2 protein is expressed at the late acute stage of infarct. Therefore, the expression of Bcl-2 protein may not protect acute cell death, but may play a role in the prevention of late cell death after myocardial is chemia-reperfusion.

연구배경 : 심근의 허혈 또는 재관류에 의한 세포사에는 괴사 이외에 세포고사가 존재함이 알려져 있다. Bcl-2 단백은 세포질에 존재하는 단백으로 세포고사를 억제하는 기능을 하며 정상심근에서는 발현되지 않으나 심근경색의 급성기에서 발현됨이 보고되어 있다. 본 연구는 가토 허혈-재관류 심근에서 Bcl-2 단백의 발현 여부와 재관류의 시간에 따른 발현의 변화를 알아보고자 하였다. 방법: 평균 무게가 2.9Kg(1.5-4.8Kg)인 가토 39마리를 이용하였다. 허혈-재관류 모델의 각 실험동물에서 좌전하행지를 30분간 결찰한 다음 1, 4, 8, 12, 24시간, 3, 7일 동안 재관류시켰다. 이후 즉시 실험동물을 희생시킨 다음 심장을 적출하여 심근조직을 얻고 10% buffered formalin에 고정하였다. Bcl-2 단백의 발현은 파라핀에 포매된 조직에서 단일클론항체를 이용한 면역조직화학적 염색으로 확인하였다. 결과: 허혈-재관류 심근 중 12, 24시간, 3일 재관류군에서 Bcl-2 단백의 발현을 관찰할 수 있었으며, 특히 24시간 재관류 심근에서 잘 관찰되었다. Bcl-2 양성염색의 심근세포는 위험부위의 구제심근에서 관찰되었다. 결론: Bcl-2 단백은 심근의 허혈-재관류에서 급성기의 비교적 후기에 발현되며, 이는 재관류 초기에서 보다는 후기에서 세포고사를 억제하는데 일부 역할을 할 것으로 사료된다.

Keywords

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