Presence of A Negative Light Regulatory Factors Binding to the cab3 Promoter of Arabidopsis Thaliana

  • Kang, Ku-Seong (Department of Genetic Engineering, College of Natural Sciences Kyungpook National University)
  • Published : 1998.12.01

Abstract

Expression of light-harvesting chlorophyll a/b-binding protein gene(cab) is repressed in the dark and activited by light. However, the detail of its regulatory mechanism is not characterized so far. To identify the interactions of cis-acting elements and trans-acting factors involvedin this regulation, nuclear extracts from the light-grown and dark-adapted Arabidopsis thaliana leaves were anlayzed for mobility shift assay against 134bp fragments had two retarded bands and one retardation band, respectively, both in light-grown and dark-adapted bands in the dark-adapted tissues. A new retardation the cab 3 expression in the dark. Several light regulatory motifs are scattered in the 146 bp region of cab 3 promoter. One of the light-regulatory motifs could be the binding site for the negative regulatory factor.

Keywords

References

  1. Biochim. Biophysic. Acta v.1184 The light-harvesting chlorophyll a/b-binding proteins Jansson, S.
  2. EMBO, J. v.5 Phytrochrome-controlled expression of a wheat cab gene in transgenic tobacco seedlings. Nagy, F.;S. A. Kay;M. Boutry;M.-Y. Hsu;N.-H. Chua
  3. Plant Physiol. v.107 Regulation of early light-inducible protein gene expression by blue and red light in etiolated seedlings involoves nuclear and plastid factors Adamska, I.
  4. Gene regulation: A eukaryotic perspective Latchman, D. S.
  5. Proc. Natl. Sci. USA v.85 Identification of upstream regulatory element involved in the developmental expression of the Arabidopsis thaliana cab1 gene Ha, S. B.;G. An
  6. Plant Mol. Biol. v.12 Structural and functional analyses of Arabidopsis thaliana chlorophyll a/b-binding protein (cab) promoters Mitra, A.;H. K. Choi;G. An
  7. Photochem. Photobiol. v.64 A negative regulatory factor for the dark repression of Arabidopsis thaliana cab1 gene Choi, D.-K;H.-J. Park;I.-S. Kim
  8. Molecular cloning : A laboratory mannual Maniatis, T.;E. F. Fritch;J. Sambrook
  9. J. Photoscience v.3 Binding of leaf nuclear protein to light-responesive elements of cab promoters of Arabidopsis thaliana Lee, H.-H.;H.-J. Park;I.-S. Kim
  10. Proc. Natl. Acad. Sci. USA v.85 An evolutionary conserved protein binding sequence upstream of a plant light-regulated gene Giuliano, G.;E. Pichersky;V. S. Malik;M. P. Timko;P. A. Scolnik;A. R. Cashmore
  11. Plant Physiol. v.65 Transcription in isolated wheat nuclei Luthe, D. S.;R. S. Quatrano
  12. Anal. Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  13. Annu. Rev. Biochem. v.58 Eukaryotic transcriptional regulatory proteins Johnson, P. F.;S. L. McKnight
  14. Methods Enzymol. v.273 Purification, characterization, and role of CCAAT-binding factor on transcription Maity, S. N.;B. D. Crombregghe
  15. J. Biol. Chem. v.272 Oct-1 and CCAAT/enhancer-binding protein (C/EBP) bind to overlapping elements within the interleukin-8 promoter Wu. G. D.;E. L. Lai;N. Huang;X. Wen
  16. Proc. Natl. Acad. Sci. USA v.94 Identification of a novel negative retinoic acid responsive element in the promoter of the human matrix Gla protein gene Kirfel. J.;M. Kelter;L. M. Cancela;P. A. Price;R. Schule
  17. EMBO J. v.15 Combinatorial interplay of promoter elements constitutes the minimal determinants for light and developmental control of gene expression in Arabidopsis Puente, P.;N. Wei;X. W. Deng
  18. Plant Cell v.6 Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter Kehoe, D. M.;J. Degenhart;I. Winicov;E. M. Tobin
  19. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.46 Light-regulated transcription Terzaghi, W. B.;A. R. Cashmore
  20. EMBO J. v.9 Photoregulated gene expression mayinvolve ubiquitous DNA binding proteins Schindler, U.;A. R. Cashmore
  21. Plant Physiol. v.108 A region of the Arabidopsis Lhcbl $^*$3 promoter that binds to CA-1 activity is essestial for high expression and phytochrome regualation Kenigsbuch, D.;E. M. Tobin
  22. Plant Cell v.1 Multiple DNA-protein complexes at a circadian-regulated promoter element. Carre, I. A.;S. A. Kay
  23. Proc. Natl. Acad. Sci. USA v.92 Functional dissection of circadian clock-and phytochrome-regulated transcription of the Arabidopsis CAB2 gene Anderson, S. L.;S. A. Kay