Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
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Presence of A Negative Light Regulatory Factors Binding to the cab3 Promoter of Arabidopsis Thaliana

  • Kang, Ku-Seong (Department of Genetic Engineering, College of Natural Sciences Kyungpook National University)
  • Published : 1998.12.01
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Abstract

Expression of light-harvesting chlorophyll a/b-binding protein gene(cab) is repressed in the dark and activited by light. However, the detail of its regulatory mechanism is not characterized so far. To identify the interactions of cis-acting elements and trans-acting factors involvedin this regulation, nuclear extracts from the light-grown and dark-adapted Arabidopsis thaliana leaves were anlayzed for mobility shift assay against 134bp fragments had two retarded bands and one retardation band, respectively, both in light-grown and dark-adapted bands in the dark-adapted tissues. A new retardation the cab 3 expression in the dark. Several light regulatory motifs are scattered in the 146 bp region of cab 3 promoter. One of the light-regulatory motifs could be the binding site for the negative regulatory factor.

Keywords

References

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