Abstract
The objective of this study was to test whether ZP drilling using a 1.48$\mu$m diode laser beam on mouse IVF embryos becomes effective the hatching and normal in vivo development, as a preliminary test for obtaining the additional proof that the 1.48$\mu$m diode laser could be used safely for human applications. The results obtained in this experiment were as follows: when the hatched rates of mouse embryos by laser ZP drilling according to the embryonic stage were examined until 72 hr (in case of blast tocyst: day 4 after IVF) or 120 hr (in case of 4-cell: day 2 after IVF) after treatment, the d data of laser drilled blastocysts (81.8%) was significantly higher than those of control (hatching blastocyst: day 4 after IVF) (54.2%) and laser drilled 4-cell embryos (45.5%) (p<0.05). When the effect of laser drilling on implantation rates following embryo transfer in day 3 synchronized pseudopregnant recipients was examined, the l laser drilled group (48.7%) was slightly higher than that of control group (43.6%). In addition, when the several pregnant mice delivered in two groups were analysed their chromosomal normality and tested reproductive ability, all p pups were presented normal chromosomal number (n=40) and showed normal growth and reproductive ability. Therefore, these results dem-onstrated that ZP drilling using a 1.48$\mu$m diode l laser can increase the embryo hatching and ind duce the normal pregnancy of mouse embryos.
본 실험은 1.48$\mu\textrm{m}$ diode laser의 인간배에 대한 적용 가능성 여부를 조사하기 위한 예비실험으로, 체외생산된 생쥐배에 1.48$\mu\textrm{m}$ diode laser를 이용한 zona pellucida (ZP) drilling 처리가 배의 부화와 체내발달에 효과적인지를 조사하고자 실시하였다. 그 결과는 다음과 같다. 발달단계가 상이한(4-세포기배, 배반포기배) 생쥐배에 laser ZP drilling 처리한 후 72시간 (배반포기배) 또는 120시간 (4-세포기배) 동안 배양하였던 바, laser ZP drilling 처리를 받은 배반포기배의 부화율(81.8%)이 대조군 (54. 2%)이나 laser ZP drilling 처리를 받은 4-세포기배 (45.5%) 보다 유의하게 높게 나타났다(p<0.05). 또한, laser ZP drilling된 배반포기배를 가임신이 유도된 대리모에 이식하였던 바, 처리군 (48.7%)의 착상율이 대조군 (43.6%)보다 약간 높게 나타났다. 한편, 임신된 대리모 일부는 분만을 유도하였던 바, 태어난 모든 새끼는 처리군에 관계없이 정상적인 염색체수 (n=40), 정상적인 성장과 생식기능을 나타내었다. 이러한 결과는 1.48$\mu\textrm{m}$ diode laser를 이용한 ZP drilling이 생쥐배의 부화를 증진시키고 정상적인 임신을 유도할 수 있어 인간배에 대한 적용 간으성을 시사한다고 하겠다.
