BMB Reports
- Volume 31 Issue 1
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- Pages.77-82
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- 1998
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- 1976-670X(eISSN)
An Efficient System for the Expression and Purification of Yeast Geranylgeranyl Protein Transferase Type I
- Kim, Hyun-Kyung (Department of Chemistry, College of Natural Sciences, Seoul National University) ;
- Kim, Young-Ah (Department of Chemistry, College of Natural Sciences, Seoul National University) ;
- Yang, Chul-Hak (Department of Chemistry, College of Natural Sciences, Seoul National University)
- Published : 1998.01.31
Abstract
To purify the geranylgeranyl protein transferase type I (GGPT-I) efficiently, a gene expression system using the pGEX-4T-1 vector was constructed. The cal1 gene, encoding the
Keywords
- Geranylgeranyl protein transferase type I;
- Glutathione S-transferase;
- Purification;
- Thrombin cleavage