The Effects of GAMIKWYBICHONGMUNGTANG on the Blood Cell, Serum and Antioxidant Effects on Serum, Brain Tissue of Mouse

가미귀비총명탕(加味歸脾聰明湯)이 노화(老化) 백서(白鼠)의 혈액변화(血液變化) 및 혈청(血淸)과 뇌조직(腦組織)의 항산화물활성(抗酸化物活性)에 미치는 영향(影響)

  • Kim In-Jae (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Tae Jeon University) ;
  • Lee Sang-Ryong (Dept. of Oriental Neuropsychiatry College of Oriental Medicine, Tae Jeon University)
  • 김인재 (대전대학교 한의과대학 신경정신과학교실) ;
  • 이상용 (대전대학교 한의과대학 신경정신과학교실)
  • Published : 1998.12.30

Abstract

This experiment was done to investigate the effects of GAMIKWYBICHONGTANG(GKCT) on the blood and brain tissues of aged rats. The experimental groups were divided into three groups and treated as follows for ten days before administration of scopolamine ; Non treated group(Normal), Distilled water feeding group (Control), GKCT feeding group(GKCT). After feeding them each, Control and GKCT were injected scopolamine for 5 days.We examined the changes of blood cell(WBC, RBC, platelet), blood serum(BUN, creatinine, glucose, uricacid), erythrocyte hemolysis, the activities of cholinesterase, and measured the amounts of malondialdehyde of the blood serum and checked the activities of catalase, SOD of the brain tissues.The results were as follows;1. GKCT showed significant increase of the number of WBC, but those of RBC and platelet didn't significantly changed in comparison with Control.2. GKCT showed significant decrease of BUN, creatinin, glucose, uric acid in blood serum in comparison with Control.3. Erythrocyte hemolysis were decreased significantly in GKCT in comparison with Control.4. About the activity of cholinesterase of blood serum, GKCT showed no significant increase in comparison with Control.5. In TBA reaction to measure the amount of MDA, oxidant material of blood serum of rats, GKCT showed significant decrease in comparison with Control.6. About the activity of catalase of brain tissue, GKCT showed no significant change in comparison with Control.7. About the activity of SOD of brain tissue, GKCT showed significant increse in comparison with Control.According to the above results, GKCT can reduce the formation of free radical and the accumulation of antioxidant materials, it is suggested that GAMIKWYBICHONGTANG(GKCT) has some effects on antiaging. It is also needed more following studies.

Keywords

References

  1. 東醫寶鑑 許浚
  2. 大韓韓醫學會誌 v.17 no.1 호桃藥針液의 抗酸化 效果에 對한 硏究 (I.호桃藥針液이 腎臟細胞에서 oxidant에 依한 損傷에 미치는 影響) 金永海;金甲成
  3. 蔘茸地黃湯이 腦組織의 酸化作用에 미치는 影響 金保경
  4. 동의신경정신과학회지 v.8 no.2 七福飮이 老化 白鼠 腦組織의 生化學的 變化에 미치는 影響 손정석;유영수
  5. 聰明湯이 老化白鼠 腦組織의 生化學的 變化와 神經細胞의 損傷에 미치는 影響 徐敏華
  6. 對譯證脈$\cdot$方藥合編 黃度淵
  7. 國譯醫方集解 蔡仁植;孟華燮
  8. 編註 醫學入門集 卷三 李천
  9. 黃帝內經靈樞解釋 洪元植(譯)
  10. 溫瞻湯이 腦組織의 酸化作用에 미치는 影響 鄭仁哲
  11. 釜山大學校 藥學硏究誌 v.29 no.2 浮萍草의 化學成分 및 抗酸化效果에 關한 硏究 이효은(외)
  12. 부산대학교 약학연구지 v.29 no.2 녹차로부터 분리된 Epicatechin 3-O-Gallate의 항산화작용 기전에 관한 연구 백봉숙(외)
  13. 大韓鍼灸學會誌 v.13 no.2 當歸 藥針液의 抗酸化 效果에 關한 硏究 安埈徹(외)
  14. 白何首烏 藥針의 抗酸化作用에 關한 實驗的 硏究 李鍾賢
  15. 抗酸化作用에 對한 杜沖葉藥針의 實驗的 硏究 成日煥
  16. 慶熙韓醫大論文集 v.18 no.2 鹿蔘地黃湯이 抗老化에 미치는 影響 蘇敬順(외)
  17. 大韓韓醫學會誌 v.17 no.1 左歸飮과 右歸飮에 依한 活性 酸素類의 消去件用과 抗酸化 酵素系의 活性 增力 效果에 對한 硏究 鄭智天
  18. 大韓韓醫學會誌 v.16 no.2 左歸飮과 右歸飮이 老化 Rat의 腦 過酸化脂質 生成 및 活性酸素 生成系 酵素 活性에 미치는 影響 尹哲浩(외)
  19. 環少丹이 腦組織의 酸化作用에 미치는 影響 徐元熙
  20. 洗心湯이 腦組織의 酸化作用에 미치는 影響 金聖賢
  21. Kor. J. Gerontol. v.2 Effect of AAPH on plasma antioxidants in rat. Lim, H. B.;Lee, D. W.;Cho, S.H.
  22. Proc. Soc. Exptl, Biol. Med. v.193 Mechanism of feed restㄱiction ; protection of cellular homeostasis. Yu, B.P.;LEE. D.W.;Marler, C.G.;Choi, J. H.
  23. Biochem. Biophys. Res. Commun. v.117 High Pressure liquid chromatographic separation of multiple form of cytochrome P-450. Bansal, S.K.;Love, J.;Gurtoo, H.L.
  24. J. Bio1. Chem. v.193 Protein measurement with the Folin phenol reagent. Lowry, O.H.;Rosebrough, N.J.;Farr, A.L.;Randall, R. J.
  25. Catalase erythrocytaire in blood serm; Exposes Annuels de Biochemie Medicale, 29ieme serie. Aebi, H.;Masson & Cie(eds)
  26. J. Biol. Chem. v.231 Superoxide dismutase, enzynmatic function for erythrocuprein (hemocuprein). McCord, J.R.;Colby. M.D.;Fridovich, I.
  27. Biostatistics ; A foundation for analysis in the health science.(third edition.) Daniel, W.W.
  28. FASEB J. v.1 Oxidents and human disease ; Some new concept. Barry, H.
  29. Biochim. Biophy. Acta. v.620 Benedetti, A.;Comporti, M.;Esterbauer, M.
  30. Biochem. biophys. Acta. v.646 Kunimoto, M.;Inoue, K.;Nojima, S.
  31. 노화 김숙희;김화영
  32. 임상병리파일 이귀녕;이종순
  33. 노화촉진 생쥐의 각종장기에서 산화성 변성과 산소라디칼 제거효소계의 활성에 관한 연구 김주섭
  34. 노화촉진 생쥐에서 산소라디칼 관련물질의 검색에 관한 연구 양재수
  35. 장수학 리정복
  36. 동의내과증상의 감별과 치료 민순실
  37. 申氏本草學 申佶求
  38. 漢藥臨床應用 李尙仁;安德均;辛民敎
  39. 嚴氏濟生方 嚴用和
  40. 醫部全書 第十冊(影印本) 古今圖書‘集成博物彙(編)
  41. 方劑學 康舜洙;盧昇鉉;李尙仁
  42. 東醫精神醫學 黃義完;金知赫
  43. 診療要鑑(下) 金定濟
  44. 이우주의 약리학강의 홍사석
  45. Pharamcol. Biochem Behav. v.48 The effects of haloperidol, scopolamine, and MK-801 on amphetamine-induced increases in ascorbic and uric acid as determined by voltammetry in vivo. Saponjic, R. M.;K. Mueller, D. Krug;P. M. Kunko.
  46. Pharm. Pharmacol. v.45 DAU 6215, a novel 5-HT sub(3)-receptor antagonist, selectively antagonizes scopolamine-induced deficit in a passive-avoidance task, but not scopolamine-induced hypermotility in rats. Brambilla, A.;A. Ghiorzi;N. Pitsicas;F. Borsini.