대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

신장(腎臟) 조직(組織)에서 제조의 항산화(抗酸化) 효과(效果)의 기전(機轉) 연구(硏究)

Underlying mechanism of antioxidant action of Holotrichia in renal tissues

  • 정지천 (동국대학교 한의과대학 내과학교실) ;
  • 문상원 (동국대학교 한의과대학 내과학교실) ;
  • 김길섭 (동국대학교 한의과대학 내과학교실)
  • Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Moon, Sang-Won (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University) ;
  • Kim, Kil-Seop (Dept. of Internal Medicine, College of Oriental Medicine, Dong-guk University)
  • 발행 : 1998.08.30
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초록

This study was carried out to examine mechanisms by which Holotrichia (HTC) produces protective effect against renal cell injury. HTC extraction (5%) prevented $H_2O_2(50mM)$-induced LDH release and lipid peroxidation in renal cortical slices. When slices were treated with 5% HTC extraction, cellular glutathione content and the superoxide dismutase activity were not altered in control and $H_2O_2$-treated tissues. When slices were treated with 50 mM $H_2O_2$, the catalase activity was significantly inhibited, which was completely restored by addition of 5% HTC. Treatment of slices with 5% HTC extraction increased the glutation peroxidase activity in $H_2O_2$-treated tissues. These results suggest that HTC prevents oxidant-induced cell injury and lipid peroxidation by increasing the activities of catalase and glutathione peroxidase in renal cortical slices.

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