Applied Biological Chemistry
- Volume 41 Issue 5
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- Pages.349-354
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- 1998
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Purification and Characterization of Protease Produced by Bacillus subtilis YG-95
Bacillus subtilis YG-95가 생산하는 protease의 정제와 특성
- Byun, Young-Gag (Department of Agricultural Chemistry, Kon-kuk University) ;
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Kim, Seong-Ho
(Department of Food Science & Technology, Tae-Gu University) ;
- Joo, Hyun-Kyu (Division of Food Resources, Sun-Moon University) ;
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Lee, Gap-Sang
(Department of Agricultural Chemistry, Won-Kwang University) ;
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Yim, Moo-Hyun
(Department of Food Science & Technology, Tae-Gu University)
- Published : 1998.08.31
Abstract
The protease produced by Bacillus subtilis YG-95 was purified by precipitating with ammonium sulfate, DEAE-sepharose 6B and Sephadex G-100 column chromatogtaphies and its purified enzymological characteritics were investigated. The molecular weight of purified protease was estimated about 43kilodalton by SDS PAGE The optimum pH and temperature for the purified protease activity were pH 10.0 and
Bacillus subtillis YG-95가 생산하는 protease를 ammonium sulfate