Applied Biological Chemistry
- 제41권1호
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- Pages.47-52
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- 1998
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
벼 HMG-CoA 환원효소의 특성연구
Characterization and Purification of a Microsomal 3-Hydroxy-3-Methylglutaryl-CoA Reductase in Rice Seedling
- Kim, Jai-Hyun (Department of Biochemistry and Bioproducts Research Center, Yonsei University) ;
- Paik, Young-Ki (Department of Biochemistry and Bioproducts Research Center, Yonsei University) ;
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Kim, Jong-Bum
(Division of Biochemistry, National Institute of Agricultural Science and Technology) ;
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Kim, Jong-Guk
(Department of Microbiology, Kyungpook National University) ;
- Hwang, Young-Soo (Division of Biochemistry, National Institute of Agricultural Science and Technology) ;
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Ha, Sun-Hwa
(Division of Biochemistry, National Institute of Agricultural Science and Technology)
- 발행 : 1998.02.28
초록
3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) 환원효소는 식물의 병원균 방어물질 (phytoalexin), 광합성 색소 (the phytol of chlorophyll), 성장 호르몬 (abscisic acid와 gibberellin 등) 및 스테롤 (phytosterol) 등의 생합성에 관여하는 주효소이다. 암조건 하에서 발아 후 4일째의 벼 유묘 microsome을 재료로 비이온성 detergent 인 Brij W-1 (final 0.4%)을 사용하여 가용화 시킨 후, DEAE-Sephadex A-50 크로마토그래피 칼럼과 hexaneagarose를 matrix로 기질인 HMG-CoA를 결합시켜 제조한 친화성 크로마토그래피 칼럼을 이용하여 세포막 결합효소인 HMG-CoA 환원효소를 정제하였다. 정제된 HMG-CoA 환원효소의 최종 회수율은 7.14% 였고, 분자량은 10% SDS-PAGE에서 55 kDa이였다 HMG-CoA 환원효소의 최적 반응 온도는
3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonic acid, the first intermediate of isoprenoid biosynthetic pathway in plants. The enzyme was solubilized with 0.4% Brij (polyoxyethylene ether) W-1 from a microsomal fraction of etiolated rice seedlings (Oryza sativa L.) in which its maximal activity was observed on the fourth day after germination. HMGR was purified to near homogeneity by employing
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