Korean Journal of Oriental Medicine (한국한의학연구원논문집)
- Volume 4 Issue 1 Serial No. 4
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- Pages.99-114
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- 1998
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- 1229-3377(pISSN)
The effect of herbal medicine on cultured cerebral cortical neurons induced by glutamate neurotoxicity
대뇌피질 신경세포에 미치는 glutamate 독성에 대한 한약재 효능연구
- Lee, Mi-Young (Aging and Regimen Team, KIOM) ;
- Kang, Bong-Joo (Aging and Regimen Team, KIOM) ;
- Yoon, Yoo-Sik (Aging and Regimen Team, KIOM) ;
- Hong, Seong-Gil (Aging and Regimen Team, KIOM) ;
- Gwag, Byoung-Joo (Dept. of Pharmacology, Ajou University School of Medicine) ;
- Cho, Dong-Wuk (Aging and Regimen Team, KIOM)
- 이미영 (한국한의학연구원 노화양생팀) ;
- 강봉주 (한국한의학연구원 노화양생팀) ;
- 윤유식 (한국한의학연구원 노화양생팀) ;
- 홍성길 (한국한의학연구원 노화양생팀) ;
- 곽병주 (아주대학교 의과대학 약리학교실) ;
- 조동욱 (한국한의학연구원 노화양생팀)
- Published : 1998.12.31
Abstract
The effect of herbal medicine on glutamate mediated neurotoxicity was studied in mouse neurons in primary culture. Immature cerebral cortex neurons (ED14) were maintained for up to 2 weeks in vitro, and we investigated the expression pattern of neuron differentiation and cytotoxicity of cell death, including LDH activity. Neuronal maturation initiated on day 7 and the susceptibility to glutamate-induced cell death was highly sensitive on Day 11 (Fig. 1). Thus, the exposure of the neurons to glutamate caused a dose