Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Isolation of Anti-Herpes Simplex Virus Type 1(HSV-1) Component from Thujae orientalis Semen

백자인(栢子仁)으로부터 항Herpes 바이러스 1형(HSV-1) 물질의 분리

  • Kang, Eun-Jung (Department of Research, Korea Institute of Oriental Medicine) ;
  • Kang, Bong-Joo (Department of Research, Korea Institute of Oriental Medicine) ;
  • Park, Kap-Joo (Department of Research, Korea Institute of Oriental Medicine) ;
  • Ko, Byoung-Seob (Department of Research, Korea Institute of Oriental Medicine) ;
  • Kim, Ho-Kyoung (Department of Research, Korea Institute of Oriental Medicine)
  • 강은정 (한국한의학연구원 연구부) ;
  • 강봉주 (한국한의학연구원 연구부) ;
  • 박갑주 (한국한의학연구원 연구부) ;
  • 고병섭 (한국한의학연구원 연구부) ;
  • 김호경 (한국한의학연구원 연구부)
  • Published : 1998.12.30
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Abstract

In order to search for anti-Herpes simplex virus type 1(HSV-1) agents, we screened 80 specimens of Korean traditional medicine by SRB assay. The methanol extracts of Thujae orientalis Semen (Cupressaceae) showed strong anti-HSV activity among samples tested. From the butanol fraction of Thujae orientalis Semen anti-HSV-1 agent was isolated by chromatographic separation using Amberlite XAD-4 and Sephadex LH-20. The structure was elucidated by spectroscopic methods, and was identified as ${\beta}-sitosterol$ (compound I). Compound I exhibited anti-HSV-1 activity with $EC_{50}$ of 0.6 mg/ml and $CC_{50}$ of 5.99 mg/ml, respectively.

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