Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Analysis of Higenamine Contents in Plants with HPLC and GC/MSD

HPLC 및 GC/MSD를 이용한 식물 중의 Higenamine 함량 분석

  • YunChoi, Hye-Sook (Natural Products Research Institute, Seoul National University) ;
  • Suh, Young-Bae (Natural Products Research Institute, Seoul National University) ;
  • Hahn, Young-Hee (Department of Chemistry, Sangmyung University) ;
  • Song, Yun-Seon (Doping Control Center, Korea Institute of Science and Technology) ;
  • Ryu, Jae-Chun (Doping Control Center, Korea Institute of Science and Technology) ;
  • Chung, Kyo-Soon (Natural Products Research Institute, Seoul National University)
  • 윤혜숙 (서울대학교 천연물과학연구소) ;
  • 서영배 (서울대학교 천연물과학연구소) ;
  • 한영희 (상명대학교 자연과학대학 화학과) ;
  • 송윤선 (한국과학기술연구원 Doping Control Center) ;
  • 류재천 (한국과학기술연구원 Doping Control Center) ;
  • 정교순 (서울대학교 천연물과학연구소)
  • Published : 1998.06.30
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Abstract

Higenamine is known as a cardiotonic principle of Aconiti tuber. The analytical procedures were established for the detection of higenamine in plants. The amounts of higenamine in several Aconiti tubers and the embryo of Nelumbo nucifera, another plant species known to contain higenamine, were determined. The $H_2O$ soluble fraction prepared from MeOH extract was first treated with AD-2 resin and then applied to either HPLC or GC/MSD systems. With HPLC, $6.4{\sim}19.2\;{\mu}g/g$ of higenamine were detected from various Aconiti tubers and $182.3\;{\mu}g/g$ of higenamine from the embryo of Nelumbo nucifera. The results obtained with GC/MSD also provided comparable data with those obtained with HPLC.

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